Detailed information of TA system TA215256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4342236..4343050
• Replicon: chromosome
• Accession: NZ_CP082824
• Organism: Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K8B79_RS21240
• Protein ID: WP_001054376.1
• Coordinates: 4342236..4342493 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: S1NWL7
• Locus tag: K8B79_RS21245
• Protein ID: WP_001540600.1
• Coordinates: 4342505..4343050 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B79_RS21220 (4337524)	4337524..4338630	+	1107	WP_001774143.1	N-acetylneuraminate epimerase	-
K8B79_RS21225 (4338695)	4338695..4339675	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K8B79_RS26570 (4339785)	4339785..4339990	+	206	Protein_4186	HNH endonuclease	-
K8B79_RS21230 (4340258)	4340258..4341498	-	1241	Protein_4187	helicase YjhR	-
K8B79_RS26575 (4341614)	4341614..4341745	+	132	WP_001309182.1	hypothetical protein	-
K8B79_RS21240 (4342236)	4342236..4342493	+	258	WP_001054376.1	YjhX family toxin	Toxin
K8B79_RS21245 (4342505)	4342505..4343050	+	546	WP_001540600.1	N-acetyltransferase	Antitoxin
K8B79_RS21250 (4343106)	4343106..4343852	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K8B79_RS21255 (4344021)	4344021..4344239	+	219	Protein_4192	hypothetical protein	-
K8B79_RS21260 (4344277)	4344277..4344393	+	117	Protein_4193	VOC family protein	-
K8B79_RS21265 (4344638)	4344638..4345759	+	1122	WP_000010829.1	M42 family metallopeptidase	-
K8B79_RS21270 (4345756)	4345756..4346034	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K8B79_RS21275 (4346046)	4346046..4347359	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4332022..4351274	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T215256 WP_001054376.1 NZ_CP082824:4342236-4342493 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T215256 NZ_CP110619:1384541-1384972 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19926.87 Da
• Isoelectric Point: 6.3277

>AT215256 WP_001540600.1 NZ_CP082824:4342505-4343050 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT215256 NZ_CP110619:1384284-1384544 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: S1NWL7