Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4188528..4189078 Replicon chromosome
Accession NZ_CP082824
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag K8B79_RS20500 Protein ID WP_001160966.1
Coordinates 4188528..4188842 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag K8B79_RS20505 Protein ID WP_000125568.1
Coordinates 4188845..4189078 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS20475 (4184203) 4184203..4185489 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
K8B79_RS20480 (4185489) 4185489..4186478 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K8B79_RS20485 (4186475) 4186475..4187296 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K8B79_RS20490 (4187299) 4187299..4187676 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
K8B79_RS20495 (4187683) 4187683..4188531 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K8B79_RS20500 (4188528) 4188528..4188842 - 315 WP_001160966.1 CcdB family protein Toxin
K8B79_RS20505 (4188845) 4188845..4189078 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K8B79_RS20510 (4189164) 4189164..4189643 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
K8B79_RS20515 (4189835) 4189835..4191697 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
K8B79_RS20520 (4191690) 4191690..4192220 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K8B79_RS20525 (4192328) 4192328..4193659 - 1332 WP_001183207.1 MFS transporter -
K8B79_RS20530 (4193718) 4193718..4194005 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T215249 WP_001160966.1 NZ_CP082824:c4188842-4188528 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T215249 NZ_CP110619:c755775-755338 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT215249 WP_000125568.1 NZ_CP082824:c4189078-4188845 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT215249 NZ_CP110619:c755228-754983 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References