Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1983128..1983959 Replicon chromosome
Accession NZ_CP082824
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K8B79_RS09655 Protein ID WP_000854814.1
Coordinates 1983128..1983502 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag K8B79_RS09660 Protein ID WP_001285586.1
Coordinates 1983591..1983959 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS09615 (1978525) 1978525..1979691 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
K8B79_RS09620 (1979810) 1979810..1980283 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
K8B79_RS09625 (1980481) 1980481..1981539 + 1059 WP_001200890.1 FUSC family protein -
K8B79_RS09630 (1981711) 1981711..1982040 + 330 WP_000450409.1 DUF496 family protein -
K8B79_RS09635 (1982140) 1982140..1982274 - 135 WP_231807309.1 EutP/PduV family microcompartment system protein -
K8B79_RS09640 (1982376) 1982376..1982522 + 147 Protein_1903 transposase domain-containing protein -
K8B79_RS09645 (1982811) 1982811..1982891 - 81 Protein_1904 hypothetical protein -
K8B79_RS09650 (1982937) 1982937..1983131 - 195 WP_000988601.1 DUF5983 family protein -
K8B79_RS09655 (1983128) 1983128..1983502 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K8B79_RS09660 (1983591) 1983591..1983959 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K8B79_RS09665 (1984033) 1984033..1984254 - 222 WP_000692323.1 DUF987 domain-containing protein -
K8B79_RS09670 (1984323) 1984323..1984799 - 477 WP_021531870.1 RadC family protein -
K8B79_RS09675 (1984815) 1984815..1985288 - 474 WP_001537423.1 antirestriction protein -
K8B79_RS09680 (1985551) 1985551..1986372 - 822 WP_001234571.1 DUF932 domain-containing protein -
K8B79_RS09685 (1986593) 1986593..1987003 - 411 WP_000846704.1 hypothetical protein -
K8B79_RS09690 (1987019) 1987019..1987695 - 677 Protein_1913 hypothetical protein -
K8B79_RS09695 (1987831) 1987831..1988901 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T215236 WP_000854814.1 NZ_CP082824:c1983502-1983128 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T215236 NZ_CP110619:363476-363778 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT215236 WP_001285586.1 NZ_CP082824:c1983959-1983591 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT215236 NZ_CP110619:363826-364047 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References