Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 944570..945224 Replicon chromosome
Accession NZ_CP082824
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag K8B79_RS04575 Protein ID WP_000244781.1
Coordinates 944817..945224 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K8B79_RS04570 Protein ID WP_000354046.1
Coordinates 944570..944836 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS04550 (940658) 940658..942091 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
K8B79_RS04555 (942136) 942136..942447 + 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
K8B79_RS04560 (942611) 942611..943270 + 660 WP_000250274.1 hemolysin III family protein -
K8B79_RS04565 (943347) 943347..944327 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
K8B79_RS04570 (944570) 944570..944836 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K8B79_RS04575 (944817) 944817..945224 + 408 WP_000244781.1 protein YgfX Toxin
K8B79_RS04580 (945264) 945264..945785 - 522 WP_001055874.1 flavodoxin FldB -
K8B79_RS04585 (945897) 945897..946793 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K8B79_RS04590 (946818) 946818..947528 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K8B79_RS04595 (947534) 947534..949267 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T215233 WP_000244781.1 NZ_CP082824:944817-945224 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T215233 NZ_CP110615:c3677867-3677454 [Rhodococcus sp. 75]
GTGACCGTGGTCGCGCTGGACACGAGCGCGGCGATTCCCTGGCTGCACGTCACCCACCGGGCGCACCGGGCCGTCCGTGC
CGCGGTCGGCGACCGGACCCCGGTGCTGACGACACACTCCCTCGCCGAGACGTACTCCGTGCTGACCCGTGCACCGGCCG
CCCTGCGGCTCGCCCCCGAGGACGCCGCCTCCGTGCTGGAGCGGGAGTTCGGCACCCCGGTCAGCCCCACCCCCGAGCTC
GCCGCGAGCCTGCCCCGCCACCTGGCCGACCGTGGGGTCAGCGGCGGCGCCACCTACGACGGGCTGGTCGCCCTGGCCGC
GTCGTCCGCCGGCACCGTCCTGCTCACCCGCGACGCCCGGGCGGTCTCGACCTACCGGACCTTGGGGACCCGCTTCGAGG
TCGTCGCGGACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT215233 WP_000354046.1 NZ_CP082824:944570-944836 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT215233 NZ_CP110615:c3678100-3677864 [Rhodococcus sp. 75]
ATGCACACCACGATCGACGCCGCCGGCCGCATCGTCATCCCCAAGGCCCTGCGGGACGCCGTGGGGCTCACCGGGGGGTC
CTCGGTGGACGTCAGCGTCTACGGCGGCGGGCTGCAGGTGACGCCGACGGCCCGCACCGCCACCCTCCGTCGGGTGGACG
GCCACCTCGTGGTGGAAGCGGCCGTCGAGTTCACCGACGACGTGCTGTTCGGCCTGATCGACGACGGTCGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References