Detailed information of TA system TA215227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5386693..5387301
• Replicon: chromosome
• Accession: NZ_CP082823
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q9I5J9
• Locus tag: K8B71_RS26090
• Protein ID: WP_003114156.1
• Coordinates: 5386693..5387040 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: K8B71_RS26095
• Protein ID: WP_003114155.1
• Coordinates: 5387050..5387301 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B71_RS26055 (K8B71_26055)	5382874..5383050	+	177	WP_023127210.1	hypothetical protein	-
K8B71_RS26060 (K8B71_26060)	5383047..5383673	+	627	WP_023127428.1	hypothetical protein	-
K8B71_RS26065 (K8B71_26065)	5383670..5384527	+	858	WP_023127429.1	DNA adenine methylase	-
K8B71_RS26070 (K8B71_26070)	5384515..5384757	+	243	WP_023127213.1	hypothetical protein	-
K8B71_RS26075 (K8B71_26075)	5384750..5384953	+	204	WP_031759157.1	hypothetical protein	-
K8B71_RS26080 (K8B71_26080)	5384946..5385149	+	204	WP_003098423.1	AlpA family phage regulatory protein	-
K8B71_RS26085 (K8B71_26085)	5385156..5386358	-	1203	WP_023127430.1	integrase family protein	-
K8B71_RS26090 (K8B71_26090)	5386693..5387040	-	348	WP_003114156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B71_RS26095 (K8B71_26095)	5387050..5387301	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K8B71_RS26100 (K8B71_26100)	5387515..5388497	-	983	Protein_5178	tyrosine-type recombinase/integrase	-
K8B71_RS26105 (K8B71_26105)	5388497..5389789	-	1293	WP_003134394.1	hypothetical protein	-
K8B71_RS26110 (K8B71_26110)	5390019..5391302	-	1284	WP_023127773.1	zonular occludens toxin domain-containing protein	-
K8B71_RS26115 (K8B71_26115)	5391306..5391662	-	357	WP_003114150.1	DUF2523 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	5350682..5412660	61978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12984.78 Da
• Isoelectric Point: 4.4212

>T215227 WP_003114156.1 NZ_CP082823:c5387040-5386693 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T215227 NZ_CP110611:817236-817598 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT215227 WP_003114155.1 NZ_CP082823:c5387301-5387050 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT215227 NZ_CP110611:817069-817239 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: Q9I5J9

Antitoxin

• AlphaFold DB: A0A0B0C355