Detailed information of TA system TA215224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4927897..4929155
• Replicon: chromosome
• Accession: NZ_CP082823
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: K8B71_RS23655
• Protein ID: WP_003086331.1
• Coordinates: 4927897..4928652 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: K8B71_RS23660
• Protein ID: WP_003086329.1
• Coordinates: 4928787..4929155 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B71_RS23645 (K8B71_23645)	4923065..4925608	+	2544	WP_003134033.1	acyl-homoserine-lactone acylase QuiP	-
K8B71_RS23650 (K8B71_23650)	4925763..4927124	-	1362	WP_003134034.1	DNA recombination protein RmuC	-
K8B71_RS23655 (K8B71_23655)	4927897..4928652	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
K8B71_RS23660 (K8B71_23660)	4928787..4929155	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
K8B71_RS23665 (K8B71_23665)	4929228..4930514	-	1287	WP_003134035.1	FAD-binding oxidoreductase	-
K8B71_RS23670 (K8B71_23670)	4930570..4932159	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
K8B71_RS23675 (K8B71_23675)	4932238..4932714	+	477	WP_003134036.1	elongation factor GreAB	-
K8B71_RS23680 (K8B71_23680)	4932717..4932992	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T215224 WP_003086331.1 NZ_CP082823:c4928652-4927897 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T215224 NZ_CP110535:c4225062-4224844 [Halomonas sp. BDJS001]
ATGCTGCTAGTTGAGTGGCGCCCCCAAGCCCGCGAATCGCTGTGGGCGATCCTCGACTATCTCAGCCAACGTAATCCCTA
CGCCGCCGAAGCACTTTATCAAGCCATTGAAGAAGCGACCGAGGCGCTACCTCAGCATCCCTACCTGTATCGCCCAGGTC
GCGTTGCGGGTACCCGAGAAGCTGTGTGCACCCCAACTATATTGTGGTTTACCGCTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT215224 WP_003086329.1 NZ_CP082823:c4929155-4928787 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT215224 NZ_CP110535:c4225234-4225049 [Halomonas sp. BDJS001]
ATGAGCACCCCACTGGATCCAATTGTCAGCGAATTTGAAACTCAGGAGCAGGCAGACAGCTACCAACGCTGGTTCTGCGA
GCGTGTGCAAGCCAGCTTGGATGATCCAAGGCCTTCAATCCCCCACGATGACGTGATGGGTGAAATGCGTGAATTGATCG
CTAAACGTCGCGATGCTGCTAGTTGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7