Detailed information of TA system TA215223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3866865..3868086
• Replicon: chromosome
• Accession: NZ_CP082823
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: K8B71_RS18475
• Protein ID: WP_003110488.1
• Coordinates: 3867508..3868086 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K8B71_RS18470
• Protein ID: WP_003132728.1
• Coordinates: 3866865..3867419 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B71_RS18445 (K8B71_18445)	3862431..3862982	+	552	WP_003132721.1	XRE family transcriptional regulator	-
K8B71_RS18450 (K8B71_18450)	3862979..3863377	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
K8B71_RS18455 (K8B71_18455)	3863390..3863713	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
K8B71_RS18460 (K8B71_18460)	3864044..3864505	+	462	WP_003088117.1	(2Fe-2S)-binding protein	-
K8B71_RS18465 (K8B71_18465)	3864510..3866705	+	2196	WP_023094771.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K8B71_RS18470 (K8B71_18470)	3866865..3867419	+	555	WP_003132728.1	helix-turn-helix transcriptional regulator	Antitoxin
K8B71_RS18475 (K8B71_18475)	3867508..3868086	+	579	WP_003110488.1	HD domain-containing protein	Toxin
K8B71_RS18480 (K8B71_18480)	3868196..3869383	-	1188	WP_003132729.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K8B71_RS18485 (K8B71_18485)	3869373..3871544	-	2172	WP_003132730.1	type I secretion system permease/ATPase	-
K8B71_RS18490 (K8B71_18490)	3871534..3872811	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T215223 WP_003110488.1 NZ_CP082823:3867508-3868086 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T215223 NZ_CP110535:3632120-3632383 [Halomonas sp. BDJS001]
GTGACATGGAAGTTGGCTTACACCAAGCAAGCTCAGAAAGACGCAAAGAAACTTGTTTCCAGCGGCCTCAAGCCAAAAGC
CCAGGAATTGTTGGCACTTATTGCAGAAGATCCCTACCGCAAGCCGCCTCCGTTTGAGAAGCTCATTGGTGATCTTGCGG
GAGCCTATTCACGACGCATCAACATTCAACATCGTCTGGTTTACCAAGTGCTCGAAGACGAGCGAGTGGTGAAAGTTTTG
AGGCTATGGAGCCACTACGAATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT215223 WP_003132728.1 NZ_CP082823:3866865-3867419 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT215223 NZ_CP110535:3631881-3632123 [Halomonas sp. BDJS001]
ATGACCGGAATTACAGCCACTGAGGCGCGCAGCAATCTTTACCGGTTGATTGATGAGACTGCCGAGTCTCACCAACCCAT
CGTCATCATGGGGAAGCGTAACAGTGCCGTCTTGGTATCCGAAGAAGATTGGTCGGCCATTCAGGAAACGCTTTACCTGC
TTTCTGTACCCGGCATGCGGGAATCGATTAGTGAAGGGATGAATACCCCTGTAGCTGAATGCGATGAGGAGCTGGACTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4