215221

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 1657137..1658832 Replicon chromosome
Accession NZ_CP082823
Organism Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag K8B71_RS07810 Protein ID WP_003130109.1
Coordinates 1657137..1658006 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q9HYA1
Locus tag K8B71_RS07815 Protein ID WP_003112902.1
Coordinates 1657999..1658832 (+) Length 278 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B71_RS07785 (K8B71_07785) 1653145..1653996 + 852 WP_023124672.1 ABC transporter ATP-binding protein -
K8B71_RS07790 (K8B71_07790) 1654037..1655044 + 1008 WP_003130107.1 ABC transporter substrate-binding protein -
K8B71_RS07795 (K8B71_07795) 1655041..1655817 + 777 WP_003112898.1 ABC transporter permease -
K8B71_RS07800 (K8B71_07800) 1655823..1656584 + 762 WP_003112899.1 SDR family oxidoreductase -
K8B71_RS07805 (K8B71_07805) 1656598..1657128 + 531 WP_003112900.1 cupin domain-containing protein -
K8B71_RS07810 (K8B71_07810) 1657137..1658006 + 870 WP_003130109.1 alpha/beta hydrolase Toxin
K8B71_RS07815 (K8B71_07815) 1657999..1658832 + 834 WP_003112902.1 IclR family transcriptional regulator Antitoxin
K8B71_RS07820 (K8B71_07820) 1658819..1659616 + 798 WP_003130112.1 SDR family oxidoreductase -
K8B71_RS07825 (K8B71_07825) 1659609..1661291 + 1683 WP_003130114.1 thiamine pyrophosphate-binding protein -
K8B71_RS07830 (K8B71_07830) 1661302..1662105 + 804 WP_003130116.1 aspartate dehydrogenase -
K8B71_RS07835 (K8B71_07835) 1662117..1663601 + 1485 WP_003130118.1 aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(41-272)

Antitoxin

Transcription regulator IclR, N-terminal

(16-66)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 30395.67 Da        Isoelectric Point: 6.8092

>T215221 WP_003130109.1 NZ_CP082823:1657137-1658006 [Pseudomonas aeruginosa]
MNMAQTHIPAPRLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Download         Length: 870 bp

>T215221 NZ_CP110535:926730-927473 [Halomonas sp. BDJS001]
GTGTGGGAAGCCTGTGGCGGCGCGCGAAATATTGAACCTATTTCCGGAACCCTTTATCGACTGGTGGAAAGTCAGGAGCA
AGTCGCAACGCTTAGCTATGTCGACACGTTAGAAGAGCAGGAGATGCTTGAAGCACTGCTTGAAACTGCTAAACCGCCTT
ACCCTGAACAGGAGCGAGACCTGCACTATCTTCTAAAAACCCCTTTTCGCTATCCGCCACTCAAATGGGGATCACGCTTT
GGCCGAACTTTTGAGCCGAGCATCTTTTATGGTGGCAAAAGCATTGTTACAACATTAGCTGAATCTGCTTTCTACCGGCT
TGTCTTCTGGGGCTCAATGGAAGGGGAGCCGGTGGCGGAGTCACTTCGCTCAGAACACACGCTCTTTTCGGTGGACTATG
CCGTTAAATGGGGAGTGAGGTTAAACGCAGGGCCCTTCATCGAACACCTTGATGTATTAAAGCAACCGGTTGATTACACC
CCGTGCCAGCAATTGGGCAGTGCGATGCGCACAGCGAACGTTGAAGCCTTTGAGTACGCCTCCGCAAGAGATGTTCAGGG
TGGTGCGTGCGTGGGCTTGTTTACACCTGAAGTGTTCAAACAGCATGAGCCAACCGACATGCGTCCATGGCTTTGCGTAA
CCAATGCCGATCAAGTGTCGTTCAAACGCAAAGATGCACTTGGTGTGTATACCTACTATCTCAATGATTTTCTGTATAAC
GGTGTGTTGCCGCTTCCTGCTTAG

Antitoxin


Download         Length: 278 a.a.        Molecular weight: 30877.26 Da        Isoelectric Point: 5.7725

>AT215221 WP_003112902.1 NZ_CP082823:1657999-1658832 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Download         Length: 834 bp

>AT215221 NZ_CP110535:926356-926727 [Halomonas sp. BDJS001]
ATGCCAGCCCTTTCTCAAACAAAGCCCACCCCGGAAATGGTGCTAGCTAAAGCAGTTTTGCGTGCTGCTGAGCAATTGGG
GTTATCGCAGTCTGATTTGGCAGAAGTGCTAGGCGTACACCGCACGACTATTACCCAACTTAAAAAAAGCCTGAAGCTTG
ACCCGAACTCTAAGCAGGGTGAACTAGCTCTGCTGCTTATTCGCTTGGCTCGAGCGTTATTCGCTCTTACCGGAGGCGAC
CATGCATGGATTCAGCATTTTATGCGAACATCCAACCGTGTTACTGGTGGCATCCCGGCCGAGCAGGTCACACGGTTGGA
GGGGCTGTTTGCGGTACTGAGATTCGTGGATGGTATGCGGGGTAAAGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB Q9HYA1

References