Detailed information of TA system TA215219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5658920..5659577
• Replicon: chromosome
• Accession: NZ_CP082822
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: K8B69_RS27015
• Protein ID: WP_003098540.1
• Coordinates: 5658920..5659102 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K8B69_RS27020
• Protein ID: WP_003124172.1
• Coordinates: 5659149..5659577 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B69_RS26985 (K8B69_26985)	5655645..5656190	-	546	WP_023114595.1	terminase small subunit	-
K8B69_RS26990 (K8B69_26990)	5656342..5657085	-	744	WP_023114596.1	hypothetical protein	-
K8B69_RS26995 (K8B69_26995)	5657082..5657552	-	471	WP_034010592.1	lysis system i-spanin subunit Rz	-
K8B69_RS27000 (K8B69_27000)	5657549..5657791	-	243	WP_023875728.1	hypothetical protein	-
K8B69_RS27005 (K8B69_27005)	5657788..5658405	-	618	WP_223107067.1	glycoside hydrolase family 19 protein	-
K8B69_RS27010 (K8B69_27010)	5658405..5658719	-	315	WP_015649339.1	phage holin, lambda family	-
K8B69_RS27015 (K8B69_27015)	5658920..5659102	+	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
K8B69_RS27020 (K8B69_27020)	5659149..5659577	+	429	WP_003124172.1	hypothetical protein	Antitoxin
K8B69_RS27025 (K8B69_27025)	5659876..5660433	-	558	WP_015980945.1	hypothetical protein	-
K8B69_RS27030 (K8B69_27030)	5660430..5660714	-	285	WP_015980944.1	hypothetical protein	-
K8B69_RS27035 (K8B69_27035)	5660711..5661400	-	690	WP_058130799.1	metallophosphoesterase	-
K8B69_RS27040 (K8B69_27040)	5661397..5661768	-	372	WP_023124260.1	hypothetical protein	-
K8B69_RS27045 (K8B69_27045)	5661765..5663195	-	1431	WP_058130801.1	replicative DNA helicase	-
K8B69_RS27050 (K8B69_27050)	5663192..5663980	-	789	WP_043503281.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5606252..5684967	78715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T215219 WP_003098540.1 NZ_CP082822:5658920-5659102 [Pseudomonas aeruginosa]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T215219 NZ_CP110535:c291785-291531 [Halomonas sp. BDJS001]
ATGAAACGAAAACACGCCAAGACACTGGCTGCTATTTTTAGCCGACCCACTCCCGGCACCATTGCCTGGAGTGATATCGA
ATCGCTATTCAAGGCGATGGGGGCAGAGATCCAGGAGCGGGAAGGGTCGCGTATTGCCGTTGTATTGTTTGATGAAGTGC
GTGTTTTTCATCGCCCGCACCCATCACCGGATACCGATAAGGGCGCAGTGGCCAGCATACGCAAATGGTTAGAAGAGAAC
GGAGAGACGCCATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15770.97 Da
• Isoelectric Point: 4.6562

>AT215219 WP_003124172.1 NZ_CP082822:5659149-5659577 [Pseudomonas aeruginosa]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKAHAISEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KVALWNEMIRRDMRKADLCRLLGIAQIQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT215219 NZ_CP110535:c291534-291199 [Halomonas sp. BDJS001]
ATGAATAACATCATGATCATTGACGGCTATCGCGCCGTCATCCAGTACGACCCCGACATTGAAATGTTCCGTGGCGAATT
CATTGGGCTGAGCGGCGGAGCCGACTTCTACGCCGACAGCGTTGCCAAACTACGCGACGAAGGCGAAATCTCGCTGCGTA
TTTTTCTGGAGGAGTGCCAGAAGCGCGGCATTAAACCCACCAAGACCTACTCTGGCAAGTTCCAGGTGCGCCTACCGGAA
GACCTACACAAACACGCCGCCGACGCCGCCACCGCCAAAGGACAAAGCCTTAATCAGTTTGTGGCCACCGCTATTGAGCA
CGAACTTTCTGTGTGA