Detailed information of TA system TA215217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5254458..5255044
• Replicon: chromosome
• Accession: NZ_CP082822
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: K8B69_RS24955
• Protein ID: WP_003120987.1
• Coordinates: 5254745..5255044 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K8B69_RS24950
• Protein ID: WP_003448662.1
• Coordinates: 5254458..5254748 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B69_RS24935 (K8B69_24935)	5250033..5251928	+	1896	WP_155713823.1	hypothetical protein	-
K8B69_RS24940 (K8B69_24940)	5251925..5253901	+	1977	WP_023093671.1	DEAD/DEAH box helicase	-
K8B69_RS24945 (K8B69_24945)	5254040..5254375	+	336	WP_022579623.1	hypothetical protein	-
K8B69_RS24950 (K8B69_24950)	5254458..5254748	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
K8B69_RS24955 (K8B69_24955)	5254745..5255044	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B69_RS24960 (K8B69_24960)	5255246..5256370	+	1125	WP_023093672.1	TcpQ domain-containing protein	-
K8B69_RS24965 (K8B69_24965)	5256370..5258079	+	1710	WP_023093673.1	PilN family type IVB pilus formation outer membrane protein	-
K8B69_RS24970 (K8B69_24970)	5258083..5259408	+	1326	WP_022579625.1	type 4b pilus protein PilO2	-
K8B69_RS24975 (K8B69_24975)	5259398..5259931	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5224358..5321930	97572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T215217 WP_003120987.1 NZ_CP082822:c5255044-5254745 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T215217 NZ_CP110534:c3857943-3857533 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGCTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAATGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT215217 WP_003448662.1 NZ_CP082822:c5254748-5254458 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT215217 NZ_CP110534:c3858190-3857924 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G8CP73