Detailed information of TA system TA215216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4915722..4916980
• Replicon: chromosome
• Accession: NZ_CP082822
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: K8B69_RS23155
• Protein ID: WP_003112528.1
• Coordinates: 4915722..4916477 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: K8B69_RS23160
• Protein ID: WP_003086329.1
• Coordinates: 4916612..4916980 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B69_RS23145 (K8B69_23145)	4910895..4913438	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
K8B69_RS23150 (K8B69_23150)	4913593..4914954	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
K8B69_RS23155 (K8B69_23155)	4915722..4916477	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
K8B69_RS23160 (K8B69_23160)	4916612..4916980	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
K8B69_RS23165 (K8B69_23165)	4917053..4918339	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
K8B69_RS23170 (K8B69_23170)	4918395..4919984	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
K8B69_RS23175 (K8B69_23175)	4920063..4920539	+	477	WP_003112531.1	hypothetical protein	-
K8B69_RS23180 (K8B69_23180)	4920542..4920817	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T215216 WP_003112528.1 NZ_CP082822:c4916477-4915722 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T215216 NZ_CP110534:c2973296-2972964 [Enterobacter roggenkampii]
ATGGAAAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGAGTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGTAACTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCGGGGACAAAAACAATGGGCGTGATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGAAACGGCATGCGGCTGGAACGTATACCTGACGCCGTTGTAAATGAGGTGCTTGCCAGGCTGGATGC
GATTCTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT215216 WP_003086329.1 NZ_CP082822:c4916980-4916612 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT215216 NZ_CP110534:c2973554-2973297 [Enterobacter roggenkampii]
ATGTATACAACTCGTCTTAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAACT
GTCGACGGACAGCGAAGTAGGCATGACCATCAATAATGGCTGCCTGATTATCGAACCTCAGAAACGGCCTCACTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCATGCTGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCAGTA
GGGAAGGAGCTCCTGTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7