Detailed information of TA system TA215215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3898223..3899444
• Replicon: chromosome
• Accession: NZ_CP082822
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: K8B69_RS18185
• Protein ID: WP_003102740.1
• Coordinates: 3898866..3899444 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K8B69_RS18180
• Protein ID: WP_003088112.1
• Coordinates: 3898223..3898777 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B69_RS18155 (K8B69_18155)	3893791..3894342	+	552	WP_004343115.1	XRE family transcriptional regulator	-
K8B69_RS18160 (K8B69_18160)	3894339..3894737	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
K8B69_RS18165 (K8B69_18165)	3894750..3895073	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
K8B69_RS18170 (K8B69_18170)	3895404..3895865	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
K8B69_RS18175 (K8B69_18175)	3895870..3898065	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K8B69_RS18180 (K8B69_18180)	3898223..3898777	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
K8B69_RS18185 (K8B69_18185)	3898866..3899444	+	579	WP_003102740.1	HD domain-containing protein	Toxin
K8B69_RS18190 (K8B69_18190)	3899553..3900740	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K8B69_RS18195 (K8B69_18195)	3900730..3902901	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
K8B69_RS18200 (K8B69_18200)	3902891..3904168	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T215215 WP_003102740.1 NZ_CP082822:3898866-3899444 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T215215 NZ_CP110534:c2826851-2826748 [Enterobacter roggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT215215 WP_003088112.1 NZ_CP082822:3898223-3898777 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT215215 NZ_CP110534:2826743-2826886 [Enterobacter roggenkampii]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0C7CS07