Detailed information of TA system TA215214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2799828..2800870
• Replicon: chromosome
• Accession: NZ_CP082822
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: K8B69_RS13385
• Protein ID: WP_003153636.1
• Coordinates: 2800295..2800870 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: K8B69_RS13380
• Protein ID: WP_003050245.1
• Coordinates: 2799828..2800298 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B69_RS13345 (K8B69_13345)	2795207..2796625	-	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-
K8B69_RS13350 (K8B69_13350)	2796615..2797526	-	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
K8B69_RS13355 (K8B69_13355)	2797523..2798215	-	693	WP_012614071.1	TIGR03746 family integrating conjugative element protein	-
K8B69_RS13360 (K8B69_13360)	2798212..2798622	-	411	WP_003821108.1	TIGR03750 family conjugal transfer protein	-
K8B69_RS13365 (K8B69_13365)	2798635..2798994	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
K8B69_RS13370 (K8B69_13370)	2799011..2799244	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
K8B69_RS13375 (K8B69_13375)	2799241..2799624	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
K8B69_RS13380 (K8B69_13380)	2799828..2800298	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
K8B69_RS13385 (K8B69_13385)	2800295..2800870	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
K8B69_RS13390 (K8B69_13390)	2800888..2801802	+	915	WP_003050256.1	AAA family ATPase	-
K8B69_RS13395 (K8B69_13395)	2801799..2802269	+	471	WP_003153638.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
K8B69_RS13400 (K8B69_13400)	2802266..2802766	+	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
K8B69_RS13405 (K8B69_13405)	2802766..2803668	+	903	WP_003153640.1	CBASS oligonucleotide cyclase	-
K8B69_RS13410 (K8B69_13410)	2803707..2804432	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2736456..2854175	117719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T215214 WP_003153636.1 NZ_CP082822:2800295-2800870 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T215214 NZ_CP110534:1661026-1661547 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGATGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT215214 WP_003050245.1 NZ_CP082822:2799828-2800298 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT215214 NZ_CP110534:1660750-1661019 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: I3TV68