Detailed information of TA system TA215211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 141849..142354
• Replicon: chromosome
• Accession: NZ_CP082822
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: K8B69_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 141849..142130 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: K8B69_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 142127..142354 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B69_RS00635 (K8B69_00635)	137100..138449	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
K8B69_RS00640 (K8B69_00640)	138498..139184	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
K8B69_RS00645 (K8B69_00645)	139285..140019	+	735	WP_003117208.1	GntR family transcriptional regulator	-
K8B69_RS00650 (K8B69_00650)	140199..140609	+	411	WP_003101225.1	aegerolysin family protein	-
K8B69_RS00655 (K8B69_00655)	140641..141549	-	909	WP_010793581.1	LysR family transcriptional regulator	-
K8B69_RS00660 (K8B69_00660)	141849..142130	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
K8B69_RS00665 (K8B69_00665)	142127..142354	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
K8B69_RS00670 (K8B69_00670)	142530..143150	-	621	WP_003101226.1	hypothetical protein	-
K8B69_RS00675 (K8B69_00675)	143251..143751	+	501	WP_003101228.1	LEA type 2 family protein	-
K8B69_RS00680 (K8B69_00680)	143824..144165	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
K8B69_RS00685 (K8B69_00685)	144247..145674	-	1428	WP_003083784.1	GABA permease	-
K8B69_RS00690 (K8B69_00690)	145843..147336	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T215211 WP_003083773.1 NZ_CP082822:c142130-141849 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T215211 NZ_CP110534:580733-581020 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGGGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGTCTTCTTGTTATTCTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT215211 WP_003083775.1 NZ_CP082822:c142354-142127 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT215211 NZ_CP110534:581007-581309 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGAGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGAAGCAGAGATTGATTACAGCGATATCCCTGCTACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9