Detailed information of TA system TA215209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5851850..5852436
• Replicon: chromosome
• Accession: NZ_CP082821
• Organism: Pseudomonas aeruginosa strain SCAID PLC1-2021 (16/222)

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: K8B72_RS28315
• Protein ID: WP_003120987.1
• Coordinates: 5852137..5852436 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K8B72_RS28310
• Protein ID: WP_003448662.1
• Coordinates: 5851850..5852140 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B72_RS28290 (K8B72_28290)	5847001..5847210	+	210	WP_003105733.1	cold-shock protein	-
K8B72_RS28295 (K8B72_28295)	5847432..5849321	+	1890	WP_016851610.1	hypothetical protein	-
K8B72_RS28300 (K8B72_28300)	5849318..5851294	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
K8B72_RS28305 (K8B72_28305)	5851435..5851779	+	345	WP_016851612.1	hypothetical protein	-
K8B72_RS28310 (K8B72_28310)	5851850..5852140	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
K8B72_RS28315 (K8B72_28315)	5852137..5852436	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B72_RS28320 (K8B72_28320)	5852638..5853762	+	1125	WP_004347965.1	TcpQ domain-containing protein	-
K8B72_RS28325 (K8B72_28325)	5853762..5855471	+	1710	WP_004347964.1	PilN family type IVB pilus formation outer membrane protein	-
K8B72_RS28330 (K8B72_28330)	5855475..5856800	+	1326	WP_004347963.1	type 4b pilus protein PilO2	-
K8B72_RS28335 (K8B72_28335)	5856790..5857323	+	534	WP_004347962.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T215209 WP_003120987.1 NZ_CP082821:c5852436-5852137 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T215209 NZ_CP110534:c253487-253200 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTATGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACGCTCAAACACGCCGGGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT215209 WP_003448662.1 NZ_CP082821:c5852140-5851850 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT215209 NZ_CP110534:c253796-253494 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAACAATGTATCCCAATGGCAGGAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73