Detailed information of TA system TA215205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4954798..4956056
• Replicon: chromosome
• Accession: NZ_CP082821
• Organism: Pseudomonas aeruginosa strain SCAID PLC1-2021 (16/222)

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: K8B72_RS23840
• Protein ID: WP_003086331.1
• Coordinates: 4954798..4955553 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: K8B72_RS23845
• Protein ID: WP_003086329.1
• Coordinates: 4955688..4956056 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B72_RS23830 (K8B72_23830)	4949966..4952509	+	2544	WP_003134033.1	acyl-homoserine-lactone acylase QuiP	-
K8B72_RS23835 (K8B72_23835)	4952664..4954025	-	1362	WP_003134034.1	DNA recombination protein RmuC	-
K8B72_RS23840 (K8B72_23840)	4954798..4955553	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
K8B72_RS23845 (K8B72_23845)	4955688..4956056	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
K8B72_RS23850 (K8B72_23850)	4956129..4957415	-	1287	WP_003134035.1	FAD-binding oxidoreductase	-
K8B72_RS23855 (K8B72_23855)	4957471..4959060	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
K8B72_RS23860 (K8B72_23860)	4959139..4959615	+	477	WP_003134036.1	elongation factor GreAB	-
K8B72_RS23865 (K8B72_23865)	4959618..4959893	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T215205 WP_003086331.1 NZ_CP082821:c4955553-4954798 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T215205 NZ_CP110533:c2812229-2812126 [Enterobacter quasiroggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT215205 WP_003086329.1 NZ_CP082821:c4956056-4955688 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT215205 NZ_CP110533:2812121-2812264 [Enterobacter quasiroggenkampii]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7