Detailed information of TA system TA215204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3884826..3886047
• Replicon: chromosome
• Accession: NZ_CP082821
• Organism: Pseudomonas aeruginosa strain SCAID PLC1-2021 (16/222)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: K8B72_RS18605
• Protein ID: WP_003110488.1
• Coordinates: 3885469..3886047 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K8B72_RS18600
• Protein ID: WP_003132728.1
• Coordinates: 3884826..3885380 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B72_RS18575 (K8B72_18575)	3880392..3880943	+	552	WP_003132721.1	XRE family transcriptional regulator	-
K8B72_RS18580 (K8B72_18580)	3880940..3881338	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
K8B72_RS18585 (K8B72_18585)	3881351..3881674	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
K8B72_RS18590 (K8B72_18590)	3882005..3882466	+	462	WP_003088117.1	(2Fe-2S)-binding protein	-
K8B72_RS18595 (K8B72_18595)	3882471..3884666	+	2196	WP_023094771.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K8B72_RS18600 (K8B72_18600)	3884826..3885380	+	555	WP_003132728.1	helix-turn-helix transcriptional regulator	Antitoxin
K8B72_RS18605 (K8B72_18605)	3885469..3886047	+	579	WP_003110488.1	HD domain-containing protein	Toxin
K8B72_RS18610 (K8B72_18610)	3886157..3887344	-	1188	WP_003132729.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K8B72_RS18615 (K8B72_18615)	3887334..3889505	-	2172	WP_003132730.1	type I secretion system permease/ATPase	-
K8B72_RS18620 (K8B72_18620)	3889495..3890772	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T215204 WP_003110488.1 NZ_CP082821:3885469-3886047 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T215204 NZ_CP110533:1686236-1686757 [Enterobacter quasiroggenkampii]
GTGGAAAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGATTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATCTGCTTAAAGACCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTATCGGGAAGTTGCTTTGAAAAAGCGATGCTGCCTTCCAGGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACCTGCAGGGACAGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAATGACCGGGCAAAACGATTTTATCTGAAGCAGGGTTTTATTCCGCTGACGGGTGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGTGTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT215204 WP_003132728.1 NZ_CP082821:3884826-3885380 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT215204 NZ_CP110533:1685960-1686229 [Enterobacter quasiroggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAACAGCATCGGCGTC
TCGTCTTAAACGAAGCATCATGGAATGCCGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4