Detailed information of TA system TA215202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1661766..1663461
• Replicon: chromosome
• Accession: NZ_CP082821
• Organism: Pseudomonas aeruginosa strain SCAID PLC1-2021 (16/222)

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: K8B72_RS07880
• Protein ID: WP_003130109.1
• Coordinates: 1661766..1662635 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: K8B72_RS07885
• Protein ID: WP_003112902.1
• Coordinates: 1662628..1663461 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B72_RS07855 (K8B72_07855)	1657774..1658625	+	852	WP_023124672.1	ABC transporter ATP-binding protein	-
K8B72_RS07860 (K8B72_07860)	1658666..1659673	+	1008	WP_003130107.1	ABC transporter substrate-binding protein	-
K8B72_RS07865 (K8B72_07865)	1659670..1660446	+	777	WP_003112898.1	ABC transporter permease	-
K8B72_RS07870 (K8B72_07870)	1660452..1661213	+	762	WP_003112899.1	SDR family oxidoreductase	-
K8B72_RS07875 (K8B72_07875)	1661227..1661757	+	531	WP_003112900.1	cupin domain-containing protein	-
K8B72_RS07880 (K8B72_07880)	1661766..1662635	+	870	WP_003130109.1	alpha/beta hydrolase	Toxin
K8B72_RS07885 (K8B72_07885)	1662628..1663461	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
K8B72_RS07890 (K8B72_07890)	1663448..1664245	+	798	WP_003130112.1	SDR family oxidoreductase	-
K8B72_RS07895 (K8B72_07895)	1664238..1665920	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
K8B72_RS07900 (K8B72_07900)	1665931..1666734	+	804	WP_003130116.1	aspartate dehydrogenase	-
K8B72_RS07905 (K8B72_07905)	1666746..1668230	+	1485	WP_003130118.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30395.67 Da
• Isoelectric Point: 6.8092

>T215202 WP_003130109.1 NZ_CP082821:1661766-1662635 [Pseudomonas aeruginosa]
MNMAQTHIPAPRLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T215202 NZ_CP110533:c262016-261714 [Enterobacter quasiroggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT215202 WP_003112902.1 NZ_CP082821:1662628-1663461 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT215202 NZ_CP110533:c261707-261420 [Enterobacter quasiroggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: Q9HYA1