215190

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2211037..2211566 Replicon chromosome
Accession NZ_CP082815
Organism Staphylococcus aureus strain SCAID WND1-2021 (598)

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag K8B78_RS11010 Protein ID WP_000621176.1
Coordinates 2211037..2211399 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag K8B78_RS11015 Protein ID WP_000948331.1
Coordinates 2211396..2211566 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B78_RS10990 (2208016) 2208016..2208786 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
K8B78_RS10995 (2208761) 2208761..2209240 - 480 WP_001190825.1 anti-sigma B factor RsbW -
K8B78_RS11000 (2209242) 2209242..2209568 - 327 WP_001052491.1 anti-sigma factor antagonist -
K8B78_RS11005 (2209687) 2209687..2210688 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
K8B78_RS11010 (2211037) 2211037..2211399 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K8B78_RS11015 (2211396) 2211396..2211566 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K8B78_RS11020 (2211651) 2211651..2212799 - 1149 WP_001281140.1 alanine racemase -
K8B78_RS11025 (2212865) 2212865..2213224 - 360 WP_000581197.1 holo-ACP synthase -
K8B78_RS11030 (2213228) 2213228..2213719 - 492 WP_001286801.1 PH domain-containing protein -
K8B78_RS11035 (2213712) 2213712..2215289 - 1578 WP_001294646.1 PH domain-containing protein -
K8B78_RS11040 (2215282) 2215282..2215761 - 480 WP_001287082.1 hypothetical protein -
K8B78_RS11045 (2215970) 2215970..2216530 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T215190 WP_000621176.1 NZ_CP082815:c2211399-2211037 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T215190 NZ_CP110527:c94533-94183 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT215190 WP_000948331.1 NZ_CP082815:c2211566-2211396 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT215190 NZ_CP110527:c94178-93876 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References