Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2084388..2084902 Replicon chromosome
Accession NZ_CP082813
Organism Staphylococcus aureus strain SCAID OTT1-2021 (597/2)

Toxin (Protein)


Gene name TsdT Uniprot ID A0A0U1MQX5
Locus tag K8B68_RS10210 Protein ID WP_000581795.1
Coordinates 2084388..2084597 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A0U1MQV0
Locus tag K8B68_RS10215 Protein ID WP_000138930.1
Coordinates 2084609..2084902 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B68_RS10190 (2079896) 2079896..2081254 - 1359 WP_000611479.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
K8B68_RS10195 (2081269) 2081269..2082339 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
K8B68_RS10200 (2082657) 2082657..2083859 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
K8B68_RS10205 (2084123) 2084123..2084260 - 138 WP_000828354.1 Lmo0850 family protein -
K8B68_RS10210 (2084388) 2084388..2084597 - 210 WP_000581795.1 heavy metal-associated domain-containing protein Toxin
K8B68_RS10215 (2084609) 2084609..2084902 - 294 WP_000138930.1 copper-sensing transcriptional repressor CsoR Antitoxin
K8B68_RS10220 (2085068) 2085068..2086552 + 1485 WP_000571562.1 cardiolipin synthase -
K8B68_RS10225 (2086580) 2086580..2087227 + 648 WP_001187625.1 HD domain-containing protein -
K8B68_RS10230 (2087777) 2087777..2088649 - 873 WP_000725802.1 membrane protein insertase YidC -
K8B68_RS10235 (2088735) 2088735..2089376 - 642 WP_000483162.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7842.83 Da        Isoelectric Point: 4.3869

>T215176 WP_000581795.1 NZ_CP082813:c2084597-2084388 [Staphylococcus aureus]
MIHQNTIYTAGIETKEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T215176 NZ_CP110523:c4594066-4593782 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11169.81 Da        Isoelectric Point: 6.2242

>AT215176 WP_000138930.1 NZ_CP082813:c2084902-2084609 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVKAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT215176 NZ_CP110523:c4594298-4594056 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0U1MQX5

Antitoxin

Source ID Structure
AlphaFold DB A0A0U1MQV0

References