Detailed information of TA system TA215175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2064645..2065174
• Replicon: chromosome
• Accession: NZ_CP082813
• Organism: Staphylococcus aureus strain SCAID OTT1-2021 (597/2)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K8B68_RS10120
• Protein ID: WP_000621175.1
• Coordinates: 2064645..2065007 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K8B68_RS10125
• Protein ID: WP_000948331.1
• Coordinates: 2065004..2065174 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B68_RS10100 (2061624)	2061624..2062394	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
K8B68_RS10105 (2062369)	2062369..2062848	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
K8B68_RS10110 (2062850)	2062850..2063176	-	327	WP_001052491.1	anti-sigma factor antagonist	-
K8B68_RS10115 (2063295)	2063295..2064296	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K8B68_RS10120 (2064645)	2064645..2065007	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K8B68_RS10125 (2065004)	2065004..2065174	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K8B68_RS10130 (2065259)	2065259..2066407	-	1149	WP_001281140.1	alanine racemase	-
K8B68_RS10135 (2066473)	2066473..2066832	-	360	WP_000581199.1	holo-ACP synthase	-
K8B68_RS10140 (2066836)	2066836..2067327	-	492	WP_001205907.1	PH domain-containing protein	-
K8B68_RS10145 (2067314)	2067314..2068897	-	1584	WP_001294637.1	PH domain-containing protein	-
K8B68_RS10150 (2068890)	2068890..2069369	-	480	WP_001287077.1	hypothetical protein	-
K8B68_RS10155 (2069577)	2069577..2070137	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T215175 WP_000621175.1 NZ_CP082813:c2065007-2064645 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T215175 NZ_CP110523:c4497448-4496915 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT215175 WP_000948331.1 NZ_CP082813:c2065174-2065004 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT215175 NZ_CP110523:c4497725-4497459 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1