Detailed information of TA system TA215161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VagC(antitoxin)
• Location: 1916936..1917360
• Replicon: chromosome
• Accession: NZ_CP082807
• Organism: Burkholderia contaminans strain SCAID TST1-2021 (11/270)

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K8B66_RS37960
• Protein ID: WP_223274361.1
• Coordinates: 1917199..1917360 (+)
• Length: 54 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A1E3FFT1
• Locus tag: K8B66_RS08780
• Protein ID: WP_046196769.1
• Coordinates: 1916936..1917202 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B66_RS08765 (K8B66_08765)	1912484..1914745	+	2262	WP_039358640.1	TonB-dependent siderophore receptor	-
K8B66_RS08770 (K8B66_08770)	1914832..1915670	+	839	Protein_1716	N(5)-hydroxyornithine transformylase PvdF	-
K8B66_RS08775 (K8B66_08775)	1915670..1916686	+	1017	WP_039358635.1	GNAT family N-acetyltransferase	-
K8B66_RS08780 (K8B66_08780)	1916936..1917202	+	267	WP_046196769.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K8B66_RS37960	1917199..1917360	+	162	WP_223274361.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8B66_RS37965	1917388..1917615	+	228	WP_223274360.1	hypothetical protein	-
K8B66_RS08790 (K8B66_08790)	1917664..1917903	-	240	Protein_1721	nucleotidyltransferase	-
K8B66_RS08795 (K8B66_08795)	1917917..1919005	-	1089	WP_249215684.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
K8B66_RS08800 (K8B66_08800)	1919325..1920488	+	1164	WP_039358627.1	M20 aminoacylase family protein	-
K8B66_RS08805 (K8B66_08805)	1920605..1920850	+	246	WP_076841742.1	type II toxin-antitoxin system ParD family antitoxin	-
K8B66_RS08810 (K8B66_08810)	1920896..1921135	+	240	WP_232490759.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5903.95 Da
• Isoelectric Point: 8.5349

>T215161 WP_223274361.1 NZ_CP082807:1917199-1917360 [Burkholderia contaminans]
VTTLYMLDTSICSFIMRERPPVVLSRLQSCVNAQHRIVVSAITYAEMRFGAAG

Nucleotide

• Length: 162 bp

>T215161 NZ_CP110515:c208220-207738 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGATGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGCAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTTATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9629.86 Da
• Isoelectric Point: 4.4819

>AT215161 WP_046196769.1 NZ_CP082807:1916936-1917202 [Burkholderia contaminans]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTCLSFASEPPADADFLAERPAVIEPGRFDLSDGGG
AAPTESGQ

Nucleotide

• Length: 267 bp

>AT215161 NZ_CP110515:c208489-208211 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACACCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1E3FFT1