Detailed information of TA system TA021516

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1139952..1140750
• Replicon: chromosome
• Accession: NC_007946
• Organism: Escherichia coli UTI89

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: UTI89_RS05540
• Protein ID: WP_001094434.1
• Coordinates: 1140376..1140750 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: UTI89_RS05535
• Protein ID: WP_001552747.1
• Coordinates: 1139952..1140329 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UTI89_RS05505	1136830..1137651	+	822	WP_001234585.1	DUF945 domain-containing protein	-
UTI89_RS05510	1137651..1137896	+	246	WP_001164966.1	hypothetical protein	-
UTI89_RS05515	1137990..1138463	+	474	WP_001542276.1	antirestriction protein	-
UTI89_RS05520	1138479..1138955	+	477	WP_001186727.1	RadC family protein	-
UTI89_RS05525	1139018..1139239	+	222	WP_000692315.1	DUF987 domain-containing protein	-
UTI89_RS05530	1139258..1139902	+	645	WP_000086759.1	hypothetical protein	-
UTI89_RS05535	1139952..1140329	+	378	WP_001552747.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
UTI89_RS05540	1140376..1140750	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
UTI89_RS05545	1140747..1141238	+	492	WP_000976859.1	hypothetical protein	-
UTI89_RS05550	1141250..1141447	+	198	WP_000839257.1	DUF957 domain-containing protein	-
UTI89_RS05555	1141540..1142382	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
UTI89_RS05565	1142853..1143791	+	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
UTI89_RS05570	1143846..1144583	+	738	WP_000283664.1	zinc-binding phosphatase	-
UTI89_RS05575	1144607..1145161	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T21516 WP_001094434.1 NC_007946:1140376-1140750 [Escherichia coli UTI89]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T21516 NC_007946:1140376-1140750 [Escherichia coli UTI89]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT21516 WP_001552747.1 NC_007946:1139952-1140329 [Escherichia coli UTI89]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT21516 NC_007946:1139952-1140329 [Escherichia coli UTI89]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: Q1RDD2