Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5420526..5421151 Replicon chromosome
Accession NZ_CP082805
Organism Klebsiella pneumoniae strain SCAID PHRX1-2021 (13/97)

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag K8B74_RS26120 Protein ID WP_012737091.1
Coordinates 5420526..5420909 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K8B74_RS26125 Protein ID WP_004150355.1
Coordinates 5420909..5421151 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B74_RS26105 (5417892) 5417892..5418794 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K8B74_RS26110 (5418791) 5418791..5419426 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K8B74_RS26115 (5419423) 5419423..5420352 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K8B74_RS26120 (5420526) 5420526..5420909 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
K8B74_RS26125 (5420909) 5420909..5421151 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K8B74_RS26130 (5421356) 5421356..5422273 + 918 WP_012737090.1 alpha/beta hydrolase -
K8B74_RS26135 (5422287) 5422287..5423228 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K8B74_RS26140 (5423273) 5423273..5423710 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K8B74_RS26145 (5423707) 5423707..5424567 - 861 WP_004146232.1 virulence factor BrkB family protein -
K8B74_RS26150 (5424561) 5424561..5425160 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T215156 WP_012737091.1 NZ_CP082805:c5420909-5420526 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215156 NZ_CP110515:44162-44449 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215156 WP_004150355.1 NZ_CP082805:c5421151-5420909 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215156 NZ_CP110515:43923-44162 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References