Detailed information of TA system TA215140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 371892..372802
• Replicon: plasmid unnamed1
• Accession: NZ_CP082800
• Organism: Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K8B70_RS27055
• Protein ID: WP_040217285.1
• Coordinates: 372332..372802 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: K8B70_RS27050
• Protein ID: WP_032413443.1
• Coordinates: 371892..372335 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B70_RS27025 (K8B70_27025)	368176..369594	+	1419	WP_040217041.1	UvrD-helicase domain-containing protein	-
K8B70_RS27030 (K8B70_27030)	369654..369980	+	327	WP_142390228.1	hypothetical protein	-
K8B70_RS27035 (K8B70_27035)	370006..370974	-	969	WP_016151349.1	IS5 family transposase	-
K8B70_RS27040 (K8B70_27040)	370994..371179	+	186	WP_071953846.1	DUF2188 domain-containing protein	-
K8B70_RS27045 (K8B70_27045)	371280..371792	+	513	Protein_390	DUF4113 domain-containing protein	-
K8B70_RS27050 (K8B70_27050)	371892..372335	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
K8B70_RS27055 (K8B70_27055)	372332..372802	+	471	WP_040217285.1	RES family NAD+ phosphorylase	Toxin
K8B70_RS27060 (K8B70_27060)	372912..373172	-	261	WP_017901409.1	hypothetical protein	-
K8B70_RS27065 (K8B70_27065)	373744..374787	-	1044	Protein_394	IS481 family transposase	-
K8B70_RS27070 (K8B70_27070)	374823..375791	-	969	WP_077268205.1	IS5 family transposase	-
K8B70_RS27075 (K8B70_27075)	376221..377405	+	1185	WP_040217080.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	formA	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..427807	427807
	-	inside	IScluster/Tn	-	-	370006..375791	5785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17601.88 Da
• Isoelectric Point: 4.5152

>T215140 WP_040217285.1 NZ_CP082800:372332-372802 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAVQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T215140 NZ_CP110512:12218-12598 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT215140 WP_032413443.1 NZ_CP082800:371892-372335 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT215140 NZ_CP110512:11997-12218 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4