Detailed information of TA system TA215139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 207417..208060
• Replicon: plasmid unnamed1
• Accession: NZ_CP082800
• Organism: Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K8B70_RS26215
• Protein ID: WP_048263659.1
• Coordinates: 207417..207833 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: K8B70_RS26220
• Protein ID: WP_001261276.1
• Coordinates: 207830..208060 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B70_RS26205 (K8B70_26205)	204602..205570	+	969	WP_004099053.1	IS5-like element IS903B family transposase	-
K8B70_RS26210 (K8B70_26210)	206498..207376	+	879	WP_009309916.1	restriction endonuclease	-
K8B70_RS26215 (K8B70_26215)	207417..207833	-	417	WP_048263659.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8B70_RS26220 (K8B70_26220)	207830..208060	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K8B70_RS26225 (K8B70_26225)	208975..209796	+	822	WP_004213562.1	hypothetical protein	-
K8B70_RS26230 (K8B70_26230)	209793..210572	+	780	WP_004213560.1	site-specific integrase	-
K8B70_RS26235 (K8B70_26235)	210717..211646	-	930	WP_004213558.1	Rpn family recombination-promoting nuclease/putative transposase	-
K8B70_RS26240 (K8B70_26240)	211950..212117	+	168	Protein_224	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	formA	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..427807	427807
	-	flank	IS/Tn	-	-	204602..205570	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15122.64 Da
• Isoelectric Point: 8.5403

>T215139 WP_048263659.1 NZ_CP082800:c207833-207417 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPASVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRV
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T215139 NZ_CP110511:4358519-4358917 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT215139 WP_001261276.1 NZ_CP082800:c208060-207830 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT215139 NZ_CP110511:4358292-4358519 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023