Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 118481..119006 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP082800 | ||
| Organism | Klebsiella pneumoniae strain SCAID TST1-2021 (15/226) | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | W8UQV6 |
| Locus tag | K8B70_RS25765 | Protein ID | WP_001568026.1 |
| Coordinates | 118481..118786 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | W8V2V6 |
| Locus tag | K8B70_RS25770 | Protein ID | WP_001568025.1 |
| Coordinates | 118788..119006 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K8B70_RS25745 (K8B70_25745) | 114951..116219 | + | 1269 | WP_019705993.1 | ISL3-like element ISKpn25 family transposase | - |
| K8B70_RS25750 (K8B70_25750) | 116554..117570 | - | 1017 | WP_020315256.1 | hypothetical protein | - |
| K8B70_RS25755 (K8B70_25755) | 117567..117890 | - | 324 | WP_022644730.1 | hypothetical protein | - |
| K8B70_RS25760 (K8B70_25760) | 117917..118312 | - | 396 | WP_072158608.1 | hypothetical protein | - |
| K8B70_RS25765 (K8B70_25765) | 118481..118786 | - | 306 | WP_001568026.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| K8B70_RS25770 (K8B70_25770) | 118788..119006 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| K8B70_RS25775 (K8B70_25775) | 119176..119421 | - | 246 | Protein_130 | hypothetical protein | - |
| K8B70_RS25780 (K8B70_25780) | 119561..120250 | + | 690 | WP_004193995.1 | hypothetical protein | - |
| K8B70_RS25785 (K8B70_25785) | 120282..120971 | - | 690 | WP_020315560.1 | RES family NAD+ phosphorylase | - |
| K8B70_RS25790 (K8B70_25790) | 121408..121656 | + | 249 | WP_000272716.1 | hypothetical protein | - |
| K8B70_RS25795 (K8B70_25795) | 121653..122225 | + | 573 | WP_020805590.1 | hypothetical protein | - |
| K8B70_RS25800 (K8B70_25800) | 122256..122750 | + | 495 | WP_020805591.1 | hypothetical protein | - |
| K8B70_RS25805 (K8B70_25805) | 122794..123162 | + | 369 | WP_004197807.1 | hypothetical protein | - |
| K8B70_RS25810 (K8B70_25810) | 123196..123399 | + | 204 | WP_004197808.1 | HHA domain-containing protein | - |
| K8B70_RS25815 (K8B70_25815) | 123413..123616 | + | 204 | WP_004197809.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | formA | mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla | 1..427807 | 427807 | |
| - | flank | IS/Tn | - | - | 114951..116219 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11645.32 Da Isoelectric Point: 5.6919
>T215138 WP_001568026.1 NZ_CP082800:c118786-118481 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T215138 NZ_CP110511:4063122-4063457 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT215138 WP_001568025.1 NZ_CP082800:c119006-118788 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT215138 NZ_CP110511:4062874-4063122 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A514EZN1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZP8 |