Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5075519..5076144 Replicon chromosome
Accession NZ_CP082799
Organism Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K8B70_RS24780 Protein ID WP_002882817.1
Coordinates 5075519..5075902 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K8B70_RS24785 Protein ID WP_004150355.1
Coordinates 5075902..5076144 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B70_RS24765 (5072885) 5072885..5073787 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K8B70_RS24770 (5073784) 5073784..5074419 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K8B70_RS24775 (5074416) 5074416..5075345 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K8B70_RS24780 (5075519) 5075519..5075902 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K8B70_RS24785 (5075902) 5075902..5076144 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K8B70_RS24790 (5076349) 5076349..5077266 + 918 WP_004178029.1 alpha/beta hydrolase -
K8B70_RS24795 (5077280) 5077280..5078221 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K8B70_RS24800 (5078266) 5078266..5078703 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K8B70_RS24805 (5078700) 5078700..5079560 - 861 WP_004146232.1 virulence factor BrkB family protein -
K8B70_RS24810 (5079554) 5079554..5080153 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215137 WP_002882817.1 NZ_CP082799:c5075902-5075519 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215137 NZ_CP110511:3927814-3928221 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215137 WP_004150355.1 NZ_CP082799:c5076144-5075902 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215137 NZ_CP110511:3927567-3927833 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References