Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3894070..3894689 Replicon chromosome
Accession NZ_CP082799
Organism Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K8B70_RS19165 Protein ID WP_002892050.1
Coordinates 3894471..3894689 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K8B70_RS19160 Protein ID WP_002892066.1
Coordinates 3894070..3894444 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B70_RS19150 (3889222) 3889222..3890415 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K8B70_RS19155 (3890438) 3890438..3893584 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K8B70_RS19160 (3894070) 3894070..3894444 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K8B70_RS19165 (3894471) 3894471..3894689 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K8B70_RS19170 (3894848) 3894848..3895414 + 567 WP_002892030.1 maltose O-acetyltransferase -
K8B70_RS19175 (3895386) 3895386..3895526 - 141 WP_004147370.1 hypothetical protein -
K8B70_RS19180 (3895547) 3895547..3896017 + 471 WP_002892026.1 YlaC family protein -
K8B70_RS19185 (3895992) 3895992..3897443 - 1452 WP_004177237.1 PLP-dependent aminotransferase family protein -
K8B70_RS19190 (3897544) 3897544..3898242 + 699 WP_004177238.1 GNAT family protein -
K8B70_RS19195 (3898239) 3898239..3898379 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K8B70_RS19200 (3898379) 3898379..3898642 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215133 WP_002892050.1 NZ_CP082799:3894471-3894689 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215133 NZ_CP110511:c2854698-2854387 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215133 WP_002892066.1 NZ_CP082799:3894070-3894444 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215133 NZ_CP110511:c2854386-2854096 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References