Detailed information of TA system TA215119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 32755..33290
• Replicon: plasmid unnamed2
• Accession: NZ_CP082798
• Organism: Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K8B77_RS26460
• Protein ID: WP_016531292.1
• Coordinates: 32755..33042 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W9BAK2
• Locus tag: K8B77_RS26465
• Protein ID: WP_016531291.1
• Coordinates: 33039..33290 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B77_RS26440 (K8B77_26440)	28554..29774	-	1221	WP_032446666.1	ISL3-like element ISKox3 family transposase	-
K8B77_RS26445 (K8B77_26445)	29935..30411	+	477	WP_016529487.1	hypothetical protein	-
K8B77_RS26450 (K8B77_26450)	30668..30955	-	288	WP_016529486.1	hypothetical protein	-
K8B77_RS26455 (K8B77_26455)	31045..31707	-	663	WP_016529485.1	division plane positioning ATPase MipZ	-
K8B77_RS26460 (K8B77_26460)	32755..33042	-	288	WP_016531292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B77_RS26465 (K8B77_26465)	33039..33290	-	252	WP_016531291.1	toxin-antitoxin stability system antitoxin protein StbD	Antitoxin
K8B77_RS26470 (K8B77_26470)	33386..34657	-	1272	WP_046041953.1	Y-family DNA polymerase	-
K8B77_RS26475 (K8B77_26475)	34657..35097	-	441	WP_046041964.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
K8B77_RS26480 (K8B77_26480)	35137..36216	+	1080	WP_016531016.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	clbI / basG	1..95203	95203
	-	flank	IS/Tn	-	-	28554..29774	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11120.16 Da
• Isoelectric Point: 10.4373

>T215119 WP_016531292.1 NZ_CP082798:c33042-32755 [Klebsiella pneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERMK

Nucleotide

• Length: 288 bp

>T215119 NZ_CP110473:1990624-1991937 [Pantoea anthophila]
ATGCAGAAGCTGATGGCCTGGATGAACGGCGAGCGGGTGGGGGTGCTGACCCGGCAGCGAAACGGCGCACATGAATTTGA
GTATGATGCAGGCTGGCTGCGCAGTCCGCTGGCGCGTCCCCTTTCCCTCTCTATGCCGCTGCAGGTCAGGCCGATCACCT
CCGCCGCCGTGATTAACTTTTTCGATAACCTGCTGCCTGACAGCCCGGCGATCCGTGACCGGATTGTGGCGCGCTATCAG
ATAAAATCCCGCCAGCCGTTTGATCTGCTGCAGGAGGTGGGACGAGACAGCGTCGGCGCGACCATGCTGCTGCCGCCGGA
GATGCCGCTGCCGCCGCTGCAGGCACGCTATGAGGTGCTGGATGAGGCGCGGCTGGAGAAGGTGTTGTCGGCCTATCAGT
CCGATATTCCGCTGGGGATGCTGCGTGAAGAGATCGATTTTCGCATCTCGGTGGCCGGCGCGCAGGAGAAAACGGCCCTG
CTGCTGACCGATGAGGGCTGGAGCATTCCGGCCGGGGCGACGCCCACCTCCCACATCATCAAGCTGCCCATCGGGGAGAT
TAAGCAGCCTGCCGCCACCCTGGATATGCGCGAAAGCGTGGAGAATGAGTATCTCTGTCTGGCGCTGGCCCGGGCGCTGG
GAATGGCGGTGCCGCAGGCGACCATTATCCGCGCCGGGGCGATGCGGGCGCTGGCGGTGGAGCGGTTTGACCGGCGCTGG
TCCCGCGATAAGACCCGGCTGATCCGGCTGCCGCAGGAGGATATCTGCCAGGCGCTGGGGCTGCCATCCTCAACCAAATA
TGAGTCAGACGGCGGGCCGGGCATAGAGACGATCATGCAGCTGCTGATGGGCTCCAGCCAGGCGATAGCGGATCGCTACC
GCTTTATGCAGTTTCAGGTTTTCCAGTGGCTGATCGGCGCAACCGATGGCCATGCAAAAAACTTCTCCCTGTTTATTAAG
CGCGGCGGCAGTTACCGGCTGACGCCATTTTATGACGTCATCTCAGCCTACCCGGTACTGGGTGGCACCGGCCTCAACAA
GCGGGCGCTGAAACTGGCGATGGGGCTGTGCGCCACCAAGGGTAAAAAGATGGCGATCGACAAAATCTATCCGCGCCATT
TTATCGCCACGGCAAAGCGCGTCAGTTTTGATCCGGCCAGTATGGAGGAGATTTTTGACTTCTTTATCCATGCTTTTCCC
CAGGCGGTGGTTTCGGTGCGGGATGCGCTGCCCGCCGATTTTCCGCAGCACATCGCCGACGCCATTTTCAGTCACTCCCT
GATCATGCTGGAGCGGTTACGGCACAGAACGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9285.68 Da
• Isoelectric Point: 4.2257

>AT215119 WP_016531291.1 NZ_CP082798:c33290-33039 [Klebsiella pneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDMDLE
KYL

Nucleotide

• Length: 252 bp

>AT215119 NZ_CP110473:1990376-1990624 [Pantoea anthophila]
ATGATATATAGTCCGATTCAGCTGGCAAACCGGCTGAAACTTATCCGCCAGCGCAACGGCTGGACGCAGAGTGAGCTGGC
AAAAAAAGTGGGCCTTAAGCAGGCGACGATCTCTCACTTTGAAAATGCACCTGACAGCACCTCACTGGCAACGCTGTTTA
AGCTGCTGCAGTCGCTGGAGCTGAGCCTGGAGGTTCAGGAAAAAGCGGCACCGGCGCCAGCGCCATTCAATGACGAGGAT
GACTGGTAA

Structures

Antitoxin

• AlphaFold DB: W9BAK2