Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16765..17408 Replicon plasmid unnamed2
Accession NZ_CP082798
Organism Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K8B77_RS26380 Protein ID WP_001044768.1
Coordinates 16765..17181 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K8B77_RS26385 Protein ID WP_001261287.1
Coordinates 17178..17408 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B77_RS26345 (K8B77_26345) 11859..12113 - 255 WP_016528790.1 hypothetical protein -
K8B77_RS26350 (K8B77_26350) 12189..12446 - 258 WP_004098928.1 hypothetical protein -
K8B77_RS26355 (K8B77_26355) 12495..12698 - 204 WP_004150739.1 HHA domain-containing protein -
K8B77_RS26360 (K8B77_26360) 12759..13253 - 495 WP_016528789.1 hypothetical protein -
K8B77_RS26365 (K8B77_26365) 13284..13850 - 567 WP_009654312.1 hypothetical protein -
K8B77_RS26370 (K8B77_26370) 13847..14110 - 264 WP_009310051.1 hypothetical protein -
K8B77_RS26375 (K8B77_26375) 14465..16603 + 2139 WP_000350635.1 AAA family ATPase -
K8B77_RS26380 (K8B77_26380) 16765..17181 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K8B77_RS26385 (K8B77_26385) 17178..17408 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K8B77_RS26390 (K8B77_26390) 17714..20833 + 3120 WP_032446663.1 hypothetical protein -
K8B77_RS26395 (K8B77_26395) 21096..22229 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - clbI / basG 1..95203 95203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T215118 WP_001044768.1 NZ_CP082798:c17181-16765 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T215118 NZ_CP110473:c1670584-1670249 [Pantoea anthophila]
ATGATACAGGGAGGTCATGATGTGGCAACGTTAAGGAACATCGACAGTTTCAGGGATGAATGGCTCAGGGACTTTTTTTT
ATATGGGAAACATTCCGGAAAAATCCCGATGACACTCTCATCAGCCCTGGCCAGAAAGCTCGATATTATTAATGCCGCGG
TAAACTATCAGGATCTGAGATCACCACCAGGCAATCGTTATGAGGAGCTGAACCCACCCCTGAAAGGATATGCAGCAATC
AGAGTCAATGAGCAATACCGGCTGATTTTCAAATGGATCGACGGTAAGGCTGTTGATCTCTATCTGGACCCGCATAGCTA
CAAAAGACACAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT215118 WP_001261287.1 NZ_CP082798:c17408-17178 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215118 NZ_CP110473:c1670164-1669832 [Pantoea anthophila]
ATGAATCAGGCGACCCGAAAACCGACCACTGTTGGCGACGTGTTACTTTACGAATACCTGGAGCCAGCTGGCCTGAAAAT
CCTTGATTTAGCAGAGATGCTGAAGGTACACCGTAACACCGTCAGCGCTCTGGTGAACAATAACAGGAAGCTGACACCGG
ATATGGCTTTCAGACTGGCTGCGGCCTTTGAGACCAGTGTGGAGTTCTGGCTTAACCTGCAGAACAGTGTTGATGTCTGG
GAAGTGATGCACGATGCACGCGCTCAGGAGGAGATCAGTCGCGTGACCCCGCTGAAGATGTTTCTGGCGCAGAAAGAGAA
TCAGGTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References