Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5241504..5242129 Replicon chromosome
Accession NZ_CP082796
Organism Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K8B77_RS25065 Protein ID WP_002882817.1
Coordinates 5241504..5241887 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K8B77_RS25070 Protein ID WP_004150355.1
Coordinates 5241887..5242129 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B77_RS25050 (5238870) 5238870..5239772 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K8B77_RS25055 (5239769) 5239769..5240404 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K8B77_RS25060 (5240401) 5240401..5241330 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K8B77_RS25065 (5241504) 5241504..5241887 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K8B77_RS25070 (5241887) 5241887..5242129 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K8B77_RS25075 (5242334) 5242334..5243251 + 918 WP_004181612.1 alpha/beta hydrolase -
K8B77_RS25080 (5243265) 5243265..5244206 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K8B77_RS25085 (5244251) 5244251..5244688 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K8B77_RS25090 (5244685) 5244685..5245545 - 861 WP_004146232.1 virulence factor BrkB family protein -
K8B77_RS25095 (5245539) 5245539..5246138 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215115 WP_002882817.1 NZ_CP082796:c5241887-5241504 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215115 NZ_CP110470:c537704-537453 [Rhodococcus opacus]
ATGCGAACCATGACCTACTCGGAGTCTCGCGCGCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAAT
CGTCATCACGCGGGCGGGTCACGAGCCGGTGGTGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACT
TGCTCAGGAGTCCGGAGAACGCCCGCCGGCTGTTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGAT
CTGGCTGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215115 WP_004150355.1 NZ_CP082796:c5242129-5241887 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215115 NZ_CP110470:c537456-537196 [Rhodococcus opacus]
ATGAAACTTGTCTGGGACGAGTCTGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CGACACCCTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAGG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGCCTATAAAATTGTCGATGATCAGATCCGCATTGCTGCCTGT
CGTTACCGCTACGGACACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References