Detailed information of TA system TA215114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4916400..4918081
• Replicon: chromosome
• Accession: NZ_CP082796
• Organism: Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: K8B77_RS23550
• Protein ID: WP_004146663.1
• Coordinates: 4916400..4917317 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: K8B77_RS23555
• Protein ID: WP_032446592.1
• Coordinates: 4917323..4918081 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B77_RS23530 (4911723)	4911723..4913243	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
K8B77_RS23535 (4913240)	4913240..4914238	+	999	WP_031590230.1	ribose ABC transporter permease	-
K8B77_RS23540 (4914269)	4914269..4915210	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
K8B77_RS23545 (4915285)	4915285..4916275	+	991	Protein_4618	carbohydrate kinase family protein	-
K8B77_RS23550 (4916400)	4916400..4917317	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
K8B77_RS23555 (4917323)	4917323..4918081	-	759	WP_032446592.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
K8B77_RS23560 (4918167)	4918167..4918895	-	729	WP_002885152.1	response regulator transcription factor	-
K8B77_RS23565 (4919168)	4919168..4921315	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
K8B77_RS23570 (4921545)	4921545..4922231	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T215114 WP_004146663.1 NZ_CP082796:c4917317-4916400 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T215114 NZ_CP110470:c537456-537196 [Rhodococcus opacus]
ATGAAACTTGTCTGGGACGAGTCTGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CGACACCCTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAGG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGCCTATAAAATTGTCGATGATCAGATCCGCATTGCTGCCTGT
CGTTACCGCTACGGACACTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28566.94 Da
• Isoelectric Point: 10.1296

>AT215114 WP_032446592.1 NZ_CP082796:c4918081-4917323 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDAFACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRFRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT215114 NZ_CP110470:c537704-537453 [Rhodococcus opacus]
ATGCGAACCATGACCTACTCGGAGTCTCGCGCGCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAAT
CGTCATCACGCGGGCGGGTCACGAGCCGGTGGTGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACT
TGCTCAGGAGTCCGGAGAACGCCCGCCGGCTGTTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGAT
CTGGCTGAATGA

Structures

Toxin

• AlphaFold DB: J2XCI1