Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4659218..4660028 Replicon chromosome
Accession NZ_CP082796
Organism Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K8B77_RS22290 Protein ID WP_004178461.1
Coordinates 4659218..4659751 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K8B77_RS22295 Protein ID WP_002887278.1
Coordinates 4659762..4660028 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B77_RS22285 (4658049) 4658049..4659170 + 1122 WP_009309849.1 cupin domain-containing protein -
K8B77_RS22290 (4659218) 4659218..4659751 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K8B77_RS22295 (4659762) 4659762..4660028 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K8B77_RS22300 (4660131) 4660131..4661564 - 1434 WP_032446518.1 Cu(+)/Ag(+) sensor histidine kinase -
K8B77_RS22305 (4661554) 4661554..4662237 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K8B77_RS22310 (4662409) 4662409..4663791 + 1383 WP_032446519.1 efflux transporter outer membrane subunit -
K8B77_RS22315 (4663809) 4663809..4664153 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T215112 WP_004178461.1 NZ_CP082796:c4659751-4659218 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215112 NZ_CP110470:c342582-342319 [Rhodococcus opacus]
ATGTCTGGGTTTGAGTTCGATCCGAACAAGAGCGAAGCGAACCTGGCCAAGCACGGCATCGATTTTCTGTCGGCGCAGGC
ACTGTGGCGGGATCCGCACCGGCTCGAGGTGCCCGCGAGAATTACGGATGAGCCGAGGTGGCTGGTGATCGGTGTGATTG
ACGGAAAATGGTGGTCCGCGGTGGTGACCTATCGTGCGGACCGGATCCGGATCATCTCGGTTCGGCGCTCACGTGATGGA
GAGGTGGCGCTTTATGAGAGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215112 WP_002887278.1 NZ_CP082796:c4660028-4659762 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215112 NZ_CP110470:c342329-342093 [Rhodococcus opacus]
ATGAGAGCTGACGAGTTCGACGAGCGTTTTGATGCCGGCGAGGATGTGACGTTCGCGCTCGATACGGCCAGGGCCCGGCG
CCCGGGTGAGGAGCAGCGGCGTGTCAATGTCGACTTCCCGGCGTGGATGGTCGCAGAGTTGGATCGTGAGGCGACTCGTC
TCGGGGTTACCCGGCAGTCGCTGATCAAGGTGTGGATCGCCGAGAGGCTCGAACGAAACGGCTCGACGGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References