Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4035305..4035924 Replicon chromosome
Accession NZ_CP082796
Organism Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K8B77_RS19335 Protein ID WP_002892050.1
Coordinates 4035706..4035924 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K8B77_RS19330 Protein ID WP_002892066.1
Coordinates 4035305..4035679 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B77_RS19320 (4030457) 4030457..4031650 + 1194 WP_223072675.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K8B77_RS19325 (4031673) 4031673..4034819 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K8B77_RS19330 (4035305) 4035305..4035679 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K8B77_RS19335 (4035706) 4035706..4035924 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K8B77_RS19340 (4036083) 4036083..4036649 + 567 WP_002892030.1 maltose O-acetyltransferase -
K8B77_RS19345 (4036621) 4036621..4036761 - 141 WP_004147370.1 hypothetical protein -
K8B77_RS19350 (4036782) 4036782..4037252 + 471 WP_002892026.1 YlaC family protein -
K8B77_RS19355 (4037227) 4037227..4038678 - 1452 WP_032445639.1 PLP-dependent aminotransferase family protein -
K8B77_RS19360 (4038779) 4038779..4039477 + 699 WP_002892021.1 GNAT family protein -
K8B77_RS19365 (4039474) 4039474..4039614 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K8B77_RS19370 (4039614) 4039614..4039877 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215111 WP_002892050.1 NZ_CP082796:4035706-4035924 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215111 NZ_CP110469:3697688-3697969 [Rhodococcus opacus]
GTGATCGAGTCGTTCGCCGACAAAGAGACCGAGCGGGTCTTTCACCGACAGTTCTCGAAGAAGCTCCCGCAGGACATTCA
GCGGATCGCGCGCCGGCGCCTCCTGTTGATCGAGGCTGCCACCGACGTCAACGACCTGAGGGTGCCCCCAGGCAACCGCC
TGGAGAAGCTCTCCGGGGACCGGTTGGGCCAGTACAGCGTTCGGATCAATGATCAGTACCGCATCTGTTTCATCTGGCGC
GCCACCGCCGCATACGACGTGGAGATCACCGACTATCACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215111 WP_002892066.1 NZ_CP082796:4035305-4035679 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215111 NZ_CP110469:3698028-3698321 [Rhodococcus opacus]
ATGACTACAGACCTGAGCCCAATTCACCCCGGCGAGATCCTCCGCGAGGACTTCCTCGAGCCGTTCGGCATCACGCAGAA
CCGCCTAGCCACGATCATCGGTGTTCCGCCCCGACGGATCAACGAGATCGTGCTCGGCAAGCGGTCGATCACCGCAGACA
CCGCGTTGCGGCTGGCCAAGGCGTTCGGCAACAGTGCGCAGTTCTGGCTCAATATCCAGGACCGCTACGAGCTCGACCTT
GCGGCCGACAAGATAGGCGACGCCCTCGCCAAGATCACGCGTCTCTCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References