Detailed information of TA system TA215109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2687501..2689089
• Replicon: chromosome
• Accession: NZ_CP082796
• Organism: Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: K8B77_RS12910
• Protein ID: WP_019705665.1
• Coordinates: 2687501..2688823 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: K8B77_RS12915
• Protein ID: WP_002904790.1
• Coordinates: 2688823..2689089 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B77_RS12895 (2682557)	2682557..2685058	+	2502	WP_032446039.1	fimbria/pilus outer membrane usher protein	-
K8B77_RS12900 (2685046)	2685046..2686050	+	1005	WP_032446038.1	fimbrial protein	-
K8B77_RS12905 (2686192)	2686192..2687484	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
K8B77_RS12910 (2687501)	2687501..2688823	-	1323	WP_019705665.1	type II toxin-antitoxin system HipA family toxin	Toxin
K8B77_RS12915 (2688823)	2688823..2689089	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
K8B77_RS12920 (2689354)	2689354..2689680	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
K8B77_RS12925 (2689677)	2689677..2690177	-	501	WP_032446037.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
K8B77_RS12930 (2690155)	2690155..2691534	-	1380	WP_032446036.1	nucleobase:cation symporter-2 family protein	-
K8B77_RS12935 (2691880)	2691880..2693034	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
K8B77_RS12940 (2693015)	2693015..2693938	-	924	WP_032436334.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5358

>T215109 WP_019705665.1 NZ_CP082796:c2688823-2687501 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T215109 NZ_CP110464:2905-3192 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT215109 WP_002904790.1 NZ_CP082796:c2689089-2688823 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT215109 NZ_CP110464:3179-3493 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8