Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 23120..23712 Replicon plasmid pKP4823_mcr
Accession NZ_CP082794
Organism Klebsiella pneumoniae strain KP4823

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag KCV57_RS26500 Protein ID WP_000520549.1
Coordinates 23120..23368 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag KCV57_RS26505 Protein ID WP_000681613.1
Coordinates 23365..23712 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCV57_RS26470 (KCV57_26470) 19997..20512 + 516 WP_001025395.1 J domain-containing protein -
KCV57_RS26475 (KCV57_26475) 20617..21159 + 543 WP_001083906.1 hypothetical protein -
KCV57_RS26480 (KCV57_26480) 21156..21407 + 252 WP_001230707.1 hypothetical protein -
KCV57_RS26485 (KCV57_26485) 21394..21621 + 228 WP_000801440.1 hypothetical protein -
KCV57_RS26490 (KCV57_26490) 22075..22374 + 300 WP_000650309.1 hypothetical protein -
KCV57_RS26495 (KCV57_26495) 22465..22815 + 351 WP_000854259.1 hypothetical protein -
KCV57_RS26500 (KCV57_26500) 23120..23368 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
KCV57_RS26505 (KCV57_26505) 23365..23712 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KCV57_RS26510 (KCV57_26510) 24073..24576 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
KCV57_RS26515 (KCV57_26515) 24580..25809 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
KCV57_RS26520 (KCV57_26520) 25886..26179 + 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
KCV57_RS26525 (KCV57_26525) 26176..26391 + 216 WP_000760375.1 EexN family lipoprotein -
KCV57_RS26530 (KCV57_26530) 26747..27367 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
KCV57_RS26535 (KCV57_26535) 27381..27707 + 327 WP_000008647.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..34685 34685


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T215096 WP_000520549.1 NZ_CP082794:23120-23368 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T215096 NZ_CP110404:3044707-3044814 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT215096 WP_000681613.1 NZ_CP082794:23365-23712 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT215096 NZ_CP110404:c3044657-3044594 [Shigella sonnei]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References