Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 21156..21621 Replicon plasmid pKP4823_mcr
Accession NZ_CP082794
Organism Klebsiella pneumoniae strain KP4823

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag KCV57_RS26480 Protein ID WP_001230707.1
Coordinates 21156..21407 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag KCV57_RS26485 Protein ID WP_000801440.1
Coordinates 21394..21621 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCV57_RS26455 (KCV57_26455) 16637..17164 - 528 WP_000577043.1 DUF2726 domain-containing protein -
KCV57_RS26460 (KCV57_26460) 17299..17703 - 405 WP_021526202.1 hypothetical protein -
KCV57_RS26465 (KCV57_26465) 17716..18582 - 867 WP_001353743.1 replication initiation protein -
KCV57_RS26470 (KCV57_26470) 19997..20512 + 516 WP_001025395.1 J domain-containing protein -
KCV57_RS26475 (KCV57_26475) 20617..21159 + 543 WP_001083906.1 hypothetical protein -
KCV57_RS26480 (KCV57_26480) 21156..21407 + 252 WP_001230707.1 hypothetical protein Toxin
KCV57_RS26485 (KCV57_26485) 21394..21621 + 228 WP_000801440.1 hypothetical protein Antitoxin
KCV57_RS26490 (KCV57_26490) 22075..22374 + 300 WP_000650309.1 hypothetical protein -
KCV57_RS26495 (KCV57_26495) 22465..22815 + 351 WP_000854259.1 hypothetical protein -
KCV57_RS26500 (KCV57_26500) 23120..23368 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
KCV57_RS26505 (KCV57_26505) 23365..23712 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
KCV57_RS26510 (KCV57_26510) 24073..24576 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
KCV57_RS26515 (KCV57_26515) 24580..25809 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
KCV57_RS26520 (KCV57_26520) 25886..26179 + 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
KCV57_RS26525 (KCV57_26525) 26176..26391 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..34685 34685


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type I toxin-antitoxin system, toxin Ldr

(1-35)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T215095 WP_001230707.1 NZ_CP082794:21156-21407 [Klebsiella pneumoniae]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T215095 NZ_CP110404:3044172-3044279 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT215095 WP_000801440.1 NZ_CP082794:21394-21621 [Klebsiella pneumoniae]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT215095 NZ_CP110404:c3044118-3044062 [Shigella sonnei]
TCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References