Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 50571..51096 Replicon plasmid pUTI89
Accession NC_007941
Organism Escherichia coli UTI89

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag UTI89_RS25445 Protein ID WP_001159871.1
Coordinates 50791..51096 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag UTI89_RS25440 Protein ID WP_000813634.1
Coordinates 50571..50789 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UTI89_RS25435 47608..48249 - 642 WP_000990667.1 hypothetical protein -
UTI89_RS25440 50571..50789 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
UTI89_RS25445 50791..51096 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
UTI89_RS25450 51097..51906 + 810 WP_000016976.1 site-specific integrase -
UTI89_RS25455 52044..52319 - 276 WP_000239529.1 hypothetical protein -
UTI89_RS25460 52313..52957 - 645 WP_000633911.1 AAA family ATPase -
UTI89_RS25465 53186..54157 + 972 WP_001103694.1 hypothetical protein -
UTI89_RS25470 54162..54554 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
UTI89_RS25475 54559..55829 - 1271 Protein_59 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114230 114230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T21509 WP_001159871.1 NC_007941:50791-51096 [Escherichia coli UTI89]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T21509 NC_007941:50791-51096 [Escherichia coli UTI89]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT21509 WP_000813634.1 NC_007941:50571-50789 [Escherichia coli UTI89]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT21509 NC_007941:50571-50789 [Escherichia coli UTI89]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References