Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3239392..3240017 Replicon chromosome
Accession NZ_CP082791
Organism Klebsiella pneumoniae strain KP4823

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KCV57_RS15645 Protein ID WP_002882817.1
Coordinates 3239392..3239775 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KCV57_RS15650 Protein ID WP_004150355.1
Coordinates 3239775..3240017 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCV57_RS15630 (3236758) 3236758..3237660 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KCV57_RS15635 (3237657) 3237657..3238292 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KCV57_RS15640 (3238289) 3238289..3239218 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KCV57_RS15645 (3239392) 3239392..3239775 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KCV57_RS15650 (3239775) 3239775..3240017 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KCV57_RS15655 (3240222) 3240222..3241139 + 918 WP_032418237.1 alpha/beta hydrolase -
KCV57_RS15660 (3241153) 3241153..3242094 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KCV57_RS15665 (3242139) 3242139..3242576 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KCV57_RS15670 (3242573) 3242573..3243433 - 861 WP_004146232.1 virulence factor BrkB family protein -
KCV57_RS15675 (3243427) 3243427..3244026 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215080 WP_002882817.1 NZ_CP082791:c3239775-3239392 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215080 NZ_CP082791:c3239775-3239392 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215080 WP_004150355.1 NZ_CP082791:c3240017-3239775 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215080 NZ_CP082791:c3240017-3239775 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References