Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2628687..2629497 Replicon chromosome
Accession NZ_CP082791
Organism Klebsiella pneumoniae strain KP4823

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KCV57_RS12735 Protein ID WP_004178461.1
Coordinates 2628687..2629220 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KCV57_RS12740 Protein ID WP_002887278.1
Coordinates 2629231..2629497 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCV57_RS12730 (2627518) 2627518..2628639 + 1122 WP_004214138.1 cupin domain-containing protein -
KCV57_RS12735 (2628687) 2628687..2629220 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KCV57_RS12740 (2629231) 2629231..2629497 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KCV57_RS12745 (2629600) 2629600..2631033 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
KCV57_RS12750 (2631023) 2631023..2631706 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KCV57_RS12755 (2631878) 2631878..2633263 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
KCV57_RS12760 (2633281) 2633281..2633625 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T215078 WP_004178461.1 NZ_CP082791:c2629220-2628687 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215078 NZ_CP110398:3028383-3028490 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215078 WP_002887278.1 NZ_CP082791:c2629497-2629231 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215078 NZ_CP110398:c3028329-3028273 [Shigella sonnei]
TCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References