Detailed information of TA system TA215067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2211570..2212099
• Replicon: chromosome
• Accession: NZ_CP082789
• Organism: Staphylococcus aureus strain HL24830

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K3B00_RS11145
• Protein ID: WP_000621175.1
• Coordinates: 2211570..2211932 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K3B00_RS11150
• Protein ID: WP_000948331.1
• Coordinates: 2211929..2212099 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3B00_RS11125 (2208549)	2208549..2209319	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
K3B00_RS11130 (2209294)	2209294..2209773	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
K3B00_RS11135 (2209775)	2209775..2210101	-	327	WP_001052491.1	anti-sigma factor antagonist	-
K3B00_RS11140 (2210220)	2210220..2211221	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K3B00_RS11145 (2211570)	2211570..2211932	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3B00_RS11150 (2211929)	2211929..2212099	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K3B00_RS11155 (2212184)	2212184..2213332	-	1149	WP_001281142.1	alanine racemase	-
K3B00_RS11160 (2213398)	2213398..2213757	-	360	WP_000581197.1	holo-ACP synthase	-
K3B00_RS11165 (2213761)	2213761..2214252	-	492	WP_001205910.1	PH domain-containing protein	-
K3B00_RS11170 (2214239)	2214239..2215822	-	1584	WP_001294626.1	PH domain-containing protein	-
K3B00_RS11175 (2215815)	2215815..2216294	-	480	WP_001287090.1	hypothetical protein	-
K3B00_RS11180 (2216503)	2216503..2217063	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T215067 WP_000621175.1 NZ_CP082789:c2211932-2211570 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T215067 NZ_CP110395:2368657-2369082 [Paraburkholderia sp. D15]
ATGAGTGAAGCCAAGGCCTTCATCGACACCAACGTCCTGCTCTATCTGTTGTCCGCCGATAGCGAAAAAGCCGATCAGGC
GGAAGCCACGGTGCGCGCCGGCGGCCGCATCAGCGTGCAGGTGCTCAACGAAATCGCCAACGTGGCCCGGCGCGAACTCC
GCATGTCGTGGGCGGACATCGACGAGGTGCTGTCGCTGATCCGCTCGCTTTGCCCCACCGATCCGCTCACGGTCGAGATT
CACGTGCGCGGCAAACAGGTCGCCGAGCGCTACGGCCTGAGCGTCTACGACTCGATGATCGTCGCTGCGGCGCTGGTTGC
CGGCTGCGAGACGCTGTATTCCGAAGATATGCAGGACGGTCTCGTCATCGACCGGCAGGTGCAGATTCGCAATCCATTCG
CCGTTCACATGGCGCCGACTCACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT215067 WP_000948331.1 NZ_CP082789:c2212099-2211929 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT215067 NZ_CP110395:2368437-2368664 [Paraburkholderia sp. D15]
ATGGTGGTATCCCGATGGGGCAATAGTCTGGCCATCCGGCTGCCTGCGGTGGTGGTGGACGCGCTGCAATTGCAGGAAGG
CGACGACATCGAGGTGGTGCTGGCCGGCGCCCGTCTCTTCGAGTTGAAGAAAAAGCCCGGCCGCGAGGATTTTCTCGCGC
GCCTGCGCGAATTTCGCGGCAAACTGCCCGCCGACTTCACCTTCGACCGGGATGACGCGAATGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1