Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 98413..99149 Replicon plasmid pMMCC7.1
Accession NZ_CP082786
Organism Klebsiella quasipneumoniae strain MMCC7

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K7176_RS25670 Protein ID WP_003026803.1
Coordinates 98667..99149 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K7176_RS25665 Protein ID WP_003026799.1
Coordinates 98413..98679 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7176_RS25645 (K7176_25645) 94621..95112 + 492 WP_032249453.1 hypothetical protein -
K7176_RS25650 (K7176_25650) 96337..96687 - 351 WP_004200891.1 hypothetical protein -
K7176_RS25655 (K7176_25655) 97139..97687 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
K7176_RS25660 (K7176_25660) 97734..98168 + 435 WP_001567367.1 cell envelope integrity protein TolA -
K7176_RS25665 (K7176_25665) 98413..98679 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K7176_RS25670 (K7176_25670) 98667..99149 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K7176_RS25675 (K7176_25675) 99308..99505 - 198 Protein_94 IS3 family transposase -
K7176_RS25680 (K7176_25680) 99619..100587 + 969 WP_077256372.1 IS5 family transposase -
K7176_RS25685 (K7176_25685) 100785..101453 + 669 WP_032249507.1 LuxR C-terminal-related transcriptional regulator -
K7176_RS25690 (K7176_25690) 101499..102479 + 981 WP_000019403.1 IS5-like element IS5 family transposase -
K7176_RS25695 (K7176_25695) 102773..103489 + 717 WP_004149658.1 phosphodiesterase MrkJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sul2 / dfrA14 / tet(D) mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..194684 194684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T215038 WP_003026803.1 NZ_CP082786:98667-99149 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T215038 NZ_CP110373:c14981-14598 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT215038 WP_003026799.1 NZ_CP082786:98413-98679 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT215038 NZ_CP110373:c15199-14978 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATCTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCACTGACAAATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References