Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2529920..2531508 Replicon chromosome
Accession NZ_CP082785
Organism Klebsiella quasipneumoniae strain MMCC7

Toxin (Protein)


Gene name hipA Uniprot ID A0A246AZS4
Locus tag K7176_RS12260 Protein ID WP_017900381.1
Coordinates 2529920..2531242 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2A5MFC9
Locus tag K7176_RS12265 Protein ID WP_004205921.1
Coordinates 2531242..2531508 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7176_RS12225 (2525002) 2525002..2525220 + 219 WP_002904808.1 ParD-like family protein -
K7176_RS12230 (2525201) 2525201..2525983 + 783 WP_049093365.1 type I methionyl aminopeptidase -
K7176_RS12235 (2526252) 2526252..2527367 + 1116 WP_049093366.1 serine hydrolase domain-containing protein -
K7176_RS12240 (2527354) 2527354..2527698 + 345 WP_004205915.1 hypothetical protein -
K7176_RS12245 (2527727) 2527727..2528260 + 534 WP_004205916.1 hypothetical protein -
K7176_RS12250 (2528263) 2528263..2528469 + 207 WP_004205917.1 helix-turn-helix transcriptional regulator -
K7176_RS12255 (2528611) 2528611..2529903 + 1293 WP_017900382.1 glycoside hydrolase family 10 protein -
K7176_RS12260 (2529920) 2529920..2531242 - 1323 WP_017900381.1 type II toxin-antitoxin system HipA family toxin Toxin
K7176_RS12265 (2531242) 2531242..2531508 - 267 WP_004205921.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K7176_RS12270 (2531791) 2531791..2532117 - 327 WP_017900379.1 hydroxyisourate hydrolase -
K7176_RS12275 (2532114) 2532114..2532614 - 501 WP_004205925.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
K7176_RS12280 (2532592) 2532592..2533971 - 1380 WP_017900377.1 nucleobase:cation symporter-2 family protein -
K7176_RS12285 (2534317) 2534317..2535471 + 1155 WP_004205928.1 FAD-dependent urate hydroxylase HpxO -
K7176_RS12290 (2535452) 2535452..2536375 - 924 WP_004205929.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaOKP-B-15 mgtC / iroE 2341507..2703718 362211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49041.33 Da        Isoelectric Point: 8.6091

>T215034 WP_017900381.1 NZ_CP082785:c2531242-2529920 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Download         Length: 1323 bp

>T215034 NZ_CP110372:2486946-2487320 [Proteus mirabilis]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9940.38 Da        Isoelectric Point: 7.1997

>AT215034 WP_004205921.1 NZ_CP082785:c2531508-2531242 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Download         Length: 267 bp

>AT215034 NZ_CP110372:2486488-2486856 [Proteus mirabilis]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246AZS4

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MFC9

References