Detailed information of TA system TA215023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 253346..254253
• Replicon: chromosome
• Accession: NZ_CP082780
• Organism: Bacillus aquiflavi strain 3H-10

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: K6959_RS01295
• Protein ID: WP_163243090.1
• Coordinates: 253495..254253 (-)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: K6959_RS01290
• Protein ID: WP_262421848.1
• Coordinates: 253346..253498 (-)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6959_RS01265 (K6959_01265)	248864..249168	+	305	Protein_241	hypothetical protein	-
K6959_RS01270 (K6959_01270)	249464..250021	+	558	WP_163243086.1	thioredoxin family protein	-
K6959_RS01275 (K6959_01275)	250241..250744	+	504	WP_163243087.1	PTS glucose transporter subunit IIA	-
K6959_RS01280 (K6959_01280)	250783..251610	+	828	WP_163243088.1	PRD domain-containing protein	-
K6959_RS01285 (K6959_01285)	251675..253051	+	1377	WP_223087422.1	N-acetylglucosamine-specific PTS transporter subunit IIBC	-
K6959_RS01290 (K6959_01290)	253346..253498	-	153	WP_262421848.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
K6959_RS01295 (K6959_01295)	253495..254253	-	759	WP_163243090.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K6959_RS01300 (K6959_01300)	254564..254929	+	366	WP_223087424.1	hypothetical protein	-
K6959_RS01305 (K6959_01305)	255120..256355	-	1236	WP_262421849.1	S8 family serine peptidase	-
K6959_RS01310 (K6959_01310)	257314..257847	+	534	WP_163243107.1	sigma-70 family RNA polymerase sigma factor	-
K6959_RS01315 (K6959_01315)	257844..258374	+	531	WP_223087425.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (8-45)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 29886.73 Da
• Isoelectric Point: 5.1721

>T215023 WP_163243090.1 NZ_CP082780:c254253-253495 [Bacillus aquiflavi]
MILFFKLFVWACVGGIVIYVFSFWKWEKSMRKKIYAIRKTWYVIYLFGAVIFWTYEPTSIFTHWQNYLIVAVIVVLVDAF
VFLSTYIKKLGHHELATDTKELLEENRDMMKSQQNKLKTFHYLLKNEPINIYYGDIEDYITGIQELLNAFGEKIDMIATI
HSYATPKEKSFVMREITNRSAAQSVLERQEVFYSDQEKLALIPMILQEHSYVLKLKSADFVTEFDYLLFTSLISMYDLIY
APFEYEEDGDEV

Nucleotide

• Length: 759 bp

>T215023 NZ_CP110369:c4683935-4683546 [Klebsiella pneumoniae]
ATGCAAACCAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTTACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACATCCATTGATATCCTCCGGGCCAGAAAGGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGTGCTCCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 6020.18 Da
• Isoelectric Point: 11.1051

>AT215023 WP_262421848.1 NZ_CP082780:c253498-253346 [Bacillus aquiflavi]
VKSTVKHMIKTKRANVSLPKYRTSPHTDQLLKENERLIDEYKKKKKKLRI

Nucleotide

• Length: 153 bp

>AT215023 NZ_CP110369:c4684350-4683991 [Klebsiella pneumoniae]
TTGTCGAAGAAAACCACGATAGCAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCGTGTTT
TGGTGCCCGACTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGCGCGAATTTTAACGGACAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA