Detailed information of TA system TA215014

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4087706..4088360
• Replicon: chromosome
• Accession: NZ_CP082777
• Organism: Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: K8B91_RS19530
• Protein ID: WP_000244781.1
• Coordinates: 4087706..4088113 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: K8B91_RS19535
• Protein ID: WP_000354046.1
• Coordinates: 4088094..4088360 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B91_RS19510 (4083663)	4083663..4085396	-	1734	WP_000813189.1	single-stranded-DNA-specific exonuclease RecJ	-
K8B91_RS19515 (4085402)	4085402..4086112	-	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K8B91_RS19520 (4086137)	4086137..4087033	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
K8B91_RS19525 (4087145)	4087145..4087666	+	522	WP_001055874.1	flavodoxin FldB	-
K8B91_RS19530 (4087706)	4087706..4088113	-	408	WP_000244781.1	protein YgfX	Toxin
K8B91_RS19535 (4088094)	4088094..4088360	-	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
K8B91_RS19540 (4088603)	4088603..4089583	+	981	WP_000886079.1	tRNA-modifying protein YgfZ	-
K8B91_RS19545 (4089660)	4089660..4090319	-	660	WP_000250274.1	hemolysin III family protein	-
K8B91_RS19550 (4090483)	4090483..4090794	-	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
K8B91_RS19555 (4090839)	4090839..4092272	+	1434	WP_001350137.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T215014 WP_000244781.1 NZ_CP082777:c4088113-4087706 [Escherichia coli O25:H1]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T215014 NZ_CP110365:c1318734-1317988 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCATGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT215014 WP_000354046.1 NZ_CP082777:c4088360-4088094 [Escherichia coli O25:H1]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT215014 NZ_CP110365:c1317988-1317818 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: S1PAM6

Antitoxin

• PDB: 6B58
• PDB: 1X6I
• PDB: 1X6J
• PDB: 6C12
• AlphaFold DB: A0A7U9QD57