Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3168809..3169640 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K8B91_RS15410 Protein ID WP_000854814.1
Coordinates 3169266..3169640 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag K8B91_RS15405 Protein ID WP_001285586.1
Coordinates 3168809..3169177 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS15370 (3163866) 3163866..3164936 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
K8B91_RS15375 (3165072) 3165072..3165749 + 678 WP_001362823.1 hypothetical protein -
K8B91_RS15380 (3165765) 3165765..3166175 + 411 WP_000846704.1 hypothetical protein -
K8B91_RS15385 (3166396) 3166396..3167217 + 822 WP_001234571.1 DUF932 domain-containing protein -
K8B91_RS15390 (3167480) 3167480..3167953 + 474 WP_000855074.1 antirestriction protein -
K8B91_RS15395 (3167969) 3167969..3168445 + 477 WP_001351157.1 RadC family protein -
K8B91_RS15400 (3168514) 3168514..3168735 + 222 WP_000692323.1 DUF987 domain-containing protein -
K8B91_RS15405 (3168809) 3168809..3169177 + 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K8B91_RS15410 (3169266) 3169266..3169640 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K8B91_RS15415 (3169637) 3169637..3169831 + 195 WP_000988601.1 DUF5983 family protein -
K8B91_RS15420 (3169877) 3169877..3169957 + 81 Protein_3003 hypothetical protein -
K8B91_RS15425 (3170246) 3170246..3170326 - 81 WP_023441679.1 hypothetical protein -
K8B91_RS15430 (3170305) 3170305..3170628 + 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
K8B91_RS15435 (3170729) 3170729..3171058 - 330 WP_000450409.1 DUF496 family protein -
K8B91_RS15440 (3171230) 3171230..3172288 - 1059 WP_001200890.1 FUSC family protein -
K8B91_RS15445 (3172486) 3172486..3172959 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
K8B91_RS15450 (3173078) 3173078..3174244 - 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T215011 WP_000854814.1 NZ_CP082777:3169266-3169640 [Escherichia coli O25:H1]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T215011 NZ_CP110364:4363211-4363861 [Aeromonas veronii]
ATGCCATATGACTACAGCGGTACCCTCAACCCCGACAAGGCGCTGATTTTTCGCATCGTACATCGCGATAATCTGCCGTG
GATCCTAGCCAATGGCCTTTGGGCAGGGAATAGTGCGGAGAAGAGCGATAGCTGGGTGCATATTGGACTAACGGAGCTTA
TTAACAAGCGCGCCCATCATCCGGTGCCGCTGGCCCCCTATGGCGTGCTCAATGACTATGTGCCATTTTACTTCACCCCA
TTTTCTCCCATGCTTAGCAATATCCACGCAGGGAGAGGAGTACCCCGGCGCGATAACGACGAGATCCTAGTCTTGGTATC
CAGTCTGCACCGCATGGCTGAGCTTGAATTAGCGTTTTTGTTTACCGATAGCCATGCCTACTATCAATGGGCCAATTTCT
ACGATGACCTGGGTGAACTGAACCGCATCGACTGGCCTATTTTGCAGCGTCGAGACTTCAAGCGTGACCCGGATGACCCT
GCAAAGTTTGAGCGGTATCAGGCAGAGGCGTTGGTGCATCAACACTGCCCCGTTGAGGCATTAGTCGGCGTGGTGTGTTA
CAACGAAGAGACAAAGGCTAAGGTTGAGCAAACGATGGTCCAATTGGGTGTCGCCCGACCGGTTTATGTTCGTTCTGGAT
GGTATTTCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT215011 WP_001285586.1 NZ_CP082777:3168809-3169177 [Escherichia coli O25:H1]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT215011 NZ_CP110364:4363861-4364934 [Aeromonas veronii]
ATGATTCGATACACCACTGGCAACCTGCTAGATGCCCCTGTAGAGGCATTGGTGAATACAGTGAACACCGTTGGTGTCAT
GGGTAAGGGCATTGCGCTGATGTTTAAAGAGCGCTTTCCCGACAACATGGCTCGCTATGCGGCTGCCTGTAAGTCAGGGC
AAGTGACCACTGGCAAGATGTTCATCACCGAAACTGGTGAGCTACTCGGCCCCCGCTGGATCATCAACTTTCCCACCAAG
CAACATTGGCGATCTCCATCCCAGATGGCATGGATTCAGGAAGGCTTACAAGATCTGCGACATTGGCTTGTGCAAAATCA
CGTCAAATCTATCGCCATCCCCCCGCTAGGAGCAGGCAATGGTGGCCTACCATGGCCTGAAGTAAAACAGGCCATTGAAG
AGGCTCTCGGTGATCTGGATATTGAGATCCTGCTGTTTGAACCCAGCAACCAGTACCAAAATGTTGCCAAACGTACTGGG
GTCGAACATCTCACACCTGCCCGCGCCTTGATTGCCGAACTGGTCCGTCGCTACTGGTTTCTTGGCATGGAGTGCAGCCT
GCTGGAAATTCAAAAACTGGCATGGTTTCTGGAGCGCTTTATTCGTCAAACGGCGCCACAAGATCCGCTGCAGTTACACT
TCACGGCACACCATTACGGGCCTTATGCCAATAATCTAGCCCACTTGCTGAATGCAATGGACGGCAGTTATCTCAAATCA
GATAAGCGGATCCCAGACAGCAAACCCTTTGATGTCATCTGGTTCAATGATGACAAAAAGCAACAACTTGCCACCTATTT
GCATACCGAGGCGCGAGCTTACTTACCCGCGCTGGAGCAAGCTGCTGCGCTTATCGACGGATTTGAGTCACCCTATGGAA
TGGAGCTGCTAGCAACCGTTGACTGGCTGGTAAACCAAGAAGGATGCGAACCAACCGTAGAGGCGCTGCGGGCAGGACTT
ATCCGTTGGCCCAAAGGTGGCAAATGGGCACAACGTAAGGCCAAGCTGTTCGATGACAAGAGCTTGGGCATTGCCCTGAC
CCGCTTGCAGCAGAGCTTCCCTGCATATCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References