Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1444052..1444670 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K8B91_RS06885 Protein ID WP_001291435.1
Coordinates 1444052..1444270 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K8B91_RS06890 Protein ID WP_000344800.1
Coordinates 1444296..1444670 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS06850 (1439340) 1439340..1439912 + 573 WP_000779831.1 YbaY family lipoprotein -
K8B91_RS06855 (1439943) 1439943..1440254 - 312 WP_000409908.1 MGMT family protein -
K8B91_RS06865 (1440633) 1440633..1440986 + 354 WP_000878140.1 DUF1428 domain-containing protein -
K8B91_RS06870 (1441028) 1441028..1442578 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K8B91_RS06875 (1442742) 1442742..1443212 - 471 WP_001304825.1 YlaC family protein -
K8B91_RS06880 (1443328) 1443328..1443879 - 552 WP_000102539.1 maltose O-acetyltransferase -
K8B91_RS06885 (1444052) 1444052..1444270 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K8B91_RS06890 (1444296) 1444296..1444670 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K8B91_RS06895 (1445215) 1445215..1448364 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
K8B91_RS06900 (1448387) 1448387..1449580 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214999 WP_001291435.1 NZ_CP082777:c1444270-1444052 [Escherichia coli O25:H1]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214999 NZ_CP110360:480043-480384 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTTATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214999 WP_000344800.1 NZ_CP082777:c1444670-1444296 [Escherichia coli O25:H1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214999 NZ_CP110360:479813-480043 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References