Detailed information of TA system TA214998

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1129502..1130300
• Replicon: chromosome
• Accession: NZ_CP082777
• Organism: Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VRA6
• Locus tag: K8B91_RS05410
• Protein ID: WP_000854730.1
• Coordinates: 1129502..1129879 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: K8B91_RS05415
• Protein ID: WP_001285481.1
• Coordinates: 1129926..1130300 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B91_RS05385 (1124758)	1124758..1126162	+	1405	Protein_1035	IS66 family transposase	-
K8B91_RS05390 (1126416)	1126416..1127549	-	1134	WP_000555410.1	IS110-like element ISEc45 family transposase	-
K8B91_RS05395 (1127610)	1127610..1127798	+	189	Protein_1037	IS66 family transposase	-
K8B91_RS05400 (1127946)	1127946..1128934	+	989	Protein_1038	IS630 family transposase	-
K8B91_RS05405 (1129013)	1129013..1129505	-	493	Protein_1039	DUF5983 family protein	-
K8B91_RS05410 (1129502)	1129502..1129879	-	378	WP_000854730.1	TA system toxin CbtA family protein	Toxin
K8B91_RS05415 (1129926)	1129926..1130300	-	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B91_RS05420 (1130350)	1130350..1130994	-	645	WP_000086763.1	hypothetical protein	-
K8B91_RS05425 (1131013)	1131013..1131234	-	222	WP_000692315.1	DUF987 domain-containing protein	-
K8B91_RS05430 (1131297)	1131297..1131773	-	477	WP_001537421.1	RadC family protein	-
K8B91_RS05435 (1131789)	1131789..1132262	-	474	WP_000855076.1	antirestriction protein	-
K8B91_RS05440 (1132356)	1132356..1132601	-	246	WP_001164966.1	hypothetical protein	-
K8B91_RS05445 (1132601)	1132601..1133422	-	822	WP_001234359.1	DUF932 domain-containing protein	-
K8B91_RS05450 (1133522)	1133522..1133755	-	234	WP_001213776.1	DUF905 family protein	-
K8B91_RS05455 (1133873)	1133873..1134325	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.21 Da
• Isoelectric Point: 7.2923

>T214998 WP_000854730.1 NZ_CP082777:c1129879-1129502 [Escherichia coli O25:H1]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T214998 NZ_CP110360:189907-190173 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT214998 WP_001285481.1 NZ_CP082777:c1130300-1129926 [Escherichia coli O25:H1]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT214998 NZ_CP110360:189644-189907 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGATGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0H2V671

Antitoxin

• AlphaFold DB: A0A0P0SQV8