Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 930615..931165 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag K8B91_RS04465 Protein ID WP_001160966.1
Coordinates 930851..931165 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag K8B91_RS04460 Protein ID WP_000125566.1
Coordinates 930615..930848 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS04435 (925688) 925688..925975 + 288 WP_000203741.1 ferredoxin-like protein FixX -
K8B91_RS04440 (926034) 926034..927365 + 1332 WP_001183207.1 MFS transporter -
K8B91_RS04445 (927473) 927473..928003 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K8B91_RS04450 (927996) 927996..929858 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
K8B91_RS04455 (930050) 930050..930529 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
K8B91_RS04460 (930615) 930615..930848 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K8B91_RS04465 (930851) 930851..931165 + 315 WP_001160966.1 CcdB family protein Toxin
K8B91_RS04470 (931162) 931162..932010 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K8B91_RS04475 (932017) 932017..932394 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
K8B91_RS04480 (932397) 932397..933218 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K8B91_RS04485 (933215) 933215..934204 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K8B91_RS04490 (934204) 934204..935490 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T214997 WP_001160966.1 NZ_CP082777:930851-931165 [Escherichia coli O25:H1]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T214997 NZ_CP110358:c38476-38201 [Enterobacter hormaechei]
ATGACACGCTACCGGACAGAATACACAAAACGGTTCGTCAAAGAGTTTAAGAAAATTGACACGGTACACCAGAGGGTAAT
TCAGAAGTACATTGAGGCGCACATCAACAACGCACTCAATCCGCGCTTGACGGGTAAAATCCTGAAAGGAGATAAAGCCG
AATTTATCCGCTGGCGTATCGGTGATTACCGTCTGATTGCCACTATTGAAGACGATAAATTGCTGGTCATTTCGTTAAGC
GTGGGACACCGCAAAGACGTGTACAAAAAGTTTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT214997 WP_000125566.1 NZ_CP082777:930615-930848 [Escherichia coli O25:H1]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT214997 NZ_CP110358:c38712-38488 [Enterobacter hormaechei]
ATGAACAAAAACATGACGAAAGCGATCCGGTTGAATGACGAAGATTTTAACCTGTTTGAGTCTTACGCCAACGCAAAAGG
GATAACCTTCAGCGAATTGTGCAAAAGCGCAGTACGGGAAAAAATCGAGGACGAATTAGATTTGCAATGTGCTAATGAAA
GCTACGCGGATTATCTCAGCGACAAAACGACAATCAGCCACGACGAACTGTTTAAATCTATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References